Of Urfs and Orfs: A Primer on How to Analyze Derived Amino Acid Sequences

Russell F. Doolittle

ISBN 10: 0935702547 / ISBN 13: 9780935702545

Verlag: University Science Books, Mill Valley, Calif., 1987

Beschreibung

Five pages have pen underlining; binding has wear and chips and is sunned/faded; former owner's surname on the half-title page. vii, [1], 103 p., illustrated [o f nr mnqns] Size: Oversize. Bestandsnummer des Verkäufers 901024

Bibliografische Details

Titel: Of Urfs and Orfs: A Primer on How to Analyze...

Verlag: University Science Books, Mill Valley, Calif.

Erscheinungsdatum: 1987

Einband: Trade Paperback

Zustand: Fair

Auflage: 2nd Printing.

Über diesen Titel

Inhaltsangabe:

In these days of facile cloning and rapid DNA sequencing, it is not uncommon for investigators to find themselves with a DNA sequence that may or may not code for a known gene product. The sequence is 'open' when read in an appropriate frame, which is to say that there is a long run of amino acid codons before the appearance of a terminator codon. How can we find out if this 'unidentified reading frame' (URF) really codes for a genuine protein, and how can we identify it if it exists? There are two general strategies, both of which can also be applied to the characterization of any 'open reading frame' (ORF), whether or not it has been 'identified'. The first and simplest approach involves computer searching and analysis; the second employs antibodies raised against synthetic peptides patterned on the sequence of the expected gene product. Both methods have been used with great success by many investigators. Each has, nonetheless, its pitfalls and frustrations. This primer is meant to guide the researcher past those obstacles as much as possible. Graduate students and researchers interested in amino acid sequencing; molecular biologists, biochemists, chemists, and biotechnologists.

Über die Autorin bzw. den Autor:

Russell F. Doolittle is an emeritus professor at the University of California, San Diego, where he has spent most of his long career. Her received his PhD in biochemistry from Harvard University in 1962. His research interests have centered on the structure and evolution of proteins in general and, more particularly, on blood clotting proteins, an interest he developed while a graduate student at Harvard. He is a member of the US National Academy of Sciences, the American Academy of Arts and Sciences, and the American Philosophical Society.