The ultimate goal of this study is establishing a protocol for protoplast isolation and culture of both rootstocks by using different experiments in this respect communis and betuleafolia pear. Also, studying the obstacles facing protoplast isolation and culture as well as utilizing of this technique in future in breeding program to produce new rootstock in pear through protoplast fusion (somatic hybridization) or genetic transformation.

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Using of biotechnology in in vitro propagation

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