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Taschenbuch. Zustand: Neu. CRISPR-Cas Methods | M. Tofazzal Islam (u. a.) | Taschenbuch | Springer Protocols Handbooks | xiv | Englisch | 2020 | Humana | EAN 9781071606186 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu.
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Taschenbuch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template.Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.
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Hardcover. Zustand: new. Hardcover. This third edition expands upon the previous two editions with new and updated methods presenting comprehensive, step-by-step protocols for recently developed techniques that were not included in the earlier volumes. Chapters feature contributions from the globally leading experts in the field, aiming to equip researchers with the practical tools necessary for successful CRISPR experimentation. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 3 aims to be a useful and practical guide for researches to help further their study in this field. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.
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Buch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template.Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.
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Hardcover. Zustand: new. Hardcover. This third edition expands upon the previous two editions with new and updated methods presenting comprehensive, step-by-step protocols for recently developed techniques that were not included in the earlier volumes. Chapters feature contributions from the globally leading experts in the field, aiming to equip researchers with the practical tools necessary for successful CRISPR experimentation. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 3 aims to be a useful and practical guide for researches to help further their study in this field. Shipping may be from our Sydney, NSW warehouse or from our UK or US warehouse, depending on stock availability.