Socol marius (4 Ergebnisse)
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Taschenbuch. Zustand: Neu. Electrochemical synchronization of cell spreading on solid surfaces | Dictyostelium discoideum: synchronization of spreading | Marius Socol | Taschenbuch | 168 S. | Englisch | 2010 | LAP LAMBERT Academic Publishing | EAN 9783843360630 | Verantwortliche Person für die EU: BoD - Books on Demand, In de Ta…rpen 42, 22848 Norderstedt, info[at]bod[dot]de | Anbieter: preigu.
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Zustand: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Socol MariusMarius Socol, PhD in Biophysics (2010) Grenoble Joseph Fourier University and PhD in Chemistry (2008) Cluj-Napoca Babes-Bolyai University: studied Physics and Chemistry at Babes Bolyai Univers…ity of Cluj-Napoca. PostDo.
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Taschenbuch. Zustand: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Cell synchronization is important for the analysis of molecular events involved in cell spreading and motility. Electrostatic interactions between cells and surfaces were investigated in order to synchronize the first step in cell adhesio…n. LimE- GFP marked Dictyostelium discoideum cells were used for fluorescent tracking of actin polymerization events. Oscillating LimE fluorescent peaks were observed for individual cells in standard phosphate buffer during spreading. At low ionic concentration (phosphate sucrose buffer 0.17 mM), cells levitate over the conductive surfaces (Indium Tin Oxide, ITO) due to electrostatic repulsion. An electrochemical device was designed in order to apply an overpotential pulse (+2.5 V/Ag,AgCl) during 0.1 s to the ITO surface. In these conditions, protons are produced by water oxidation, which reduce the ITO negative surface charge and thus, attracting the levitating cells simultaneously. Consequently, these irreversible contacts with the surface triggered the onset of cell spreading. Therefore, we obtained synchronization of the spreading of a cell population for the first time thanks to an electrochemical method.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 168 pp. Englisch.
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Taschenbuch. Zustand: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Cell synchronization is important for the analysis of molecular events involved in cell spreading and motility. Electrostatic interactions between cells and surfaces were investigated in order to synchronize the first step in cell adhesion…. LimE- GFP marked Dictyostelium discoideum cells were used for fluorescent tracking of actin polymerization events. Oscillating LimE fluorescent peaks were observed for individual cells in standard phosphate buffer during spreading. At low ionic concentration (phosphate sucrose buffer 0.17 mM), cells levitate over the conductive surfaces (Indium Tin Oxide, ITO) due to electrostatic repulsion. An electrochemical device was designed in order to apply an overpotential pulse (+2.5 V/Ag,AgCl) during 0.1 s to the ITO surface. In these conditions, protons are produced by water oxidation, which reduce the ITO negative surface charge and thus, attracting the levitating cells simultaneously. Consequently, these irreversible contacts with the surface triggered the onset of cell spreading. Therefore, we obtained synchronization of the spreading of a cell population for the first time thanks to an electrochemical method.
