ISBN: 9781588291370 - g protein signaling: methods and protocols (methods in molecular biology, 237, band 237) (24 Ergebnisse)

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Zustand: New. A collection of methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. Editor(s): Smrcka, Alan V. Series: Methods in Molecular Biology. Num Pages: 250 pages, biography. BIC Classification: PDN; PSD. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 235 x 155 x 15. Weight in Grams: 1210. . 2003. Hardback. . . . . …

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Gebunden. Zustand: New. Includes supplementary material: sn.pub/extrasPart I. Purification of G Protein Subunits and G Protein Effectors Purification of Recombinant G Protein a Subunits from Escherichia coli Wendy K. Greentree and Maurine E. Linder Purification of G P.

Zustand: New. A collection of methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. Editor(s): Smrcka, Alan V. Series: Methods in Molecular Biology. Num Pages: 250 pages, biography. BIC Classification: PDN; PSD. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 235 x 155 x 15. Weight in Grams: 1210. . 2003. Hardback. . . . . Books ship from the US and Ireland. …

Buch. Zustand: Neu. Neuware - G proteins and G protein-coupled receptors are ubiquitously expressed proteins that regulate a wide range of physiological processes and are consequently the targets of many pharmaceuticals. In G Protein Signaling: Methods and Protocols, leading researchers describe in detail key methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. The authors focus on the mechanisms of G protein and G protein-coupled receptor function and the roles of G protein subunits in cell biology and disease. Among the readily reproducible techniques presented are those for the purification of G proteins and effector enzymes, assays of these purified G proteins and effector enzymes, and for the study of G protein interactions with effectors in intact cells. Additional methods are provided for assaying G protein-coupled receptor structure, function, and localization, for studying the physiological roles for endogenous G proteins, and for examining lipid and phosphate modifications of RGS proteins. Each fully tested protocol includes a background introduction explaining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. Comprehensive and highly practical, G Protein Signaling: Methods and Protocols offers novice and expert researchers alike an array of cutting-edge tools to illuminate the roles of G protein and G protein-coupled receptors, as well as a better understanding of their action with drugs, neurotransmitters, and sensory stimuli.…