Isbn: 9786131541681 - photoluminescent diamond nanoparticles as labels in cells: study of the nanodiamonds optical properties and investigation of their cellular uptake mechanism (omn.univ.europ.) (14 Ergebnisse)

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Taschenbuch. Zustand: Neu. Photoluminescent diamond nanoparticles as labels in cells | study of the nanodiamonds optical properties and investigation of their cellular uptake mechanism | Orestis Faklaris | Taschenbuch | 196 S. | Englisch | 2010 | Éditions universitaires européennes | EAN 9786131541681 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu.…

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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This work studies the use of Photoluminescent NanoDiamonds (PNDs) for bio-imaging applications. The work is divided in two parts. The first part concerns the study of the optical properties of nitrogen-vacancy (NV) color centers and the optimization of the NV center concentration in nanodiamonds. To enhance the imaging contrast of internalized by cells PNDs, we applied time gated imaging and studied the 2-photon excitation properties of NV centers. While implementing a pulsed excitation laser, we discovered that simultaneous one and two-photon excitation quenches the signal of PNDs. We examined how this effect can serve for super-resolution imaging of NV centers. The second part of the work is devoted to the applications of PNDs as bio-imaging probes. In the prospect of applications of PNDs as drug delivery vehicles, we studied the uptake and elucidated the intracellular localization of PNDs by blocking different entry mechanisms and by immunofluorescence experiments. Moreover we ensured that PNDs are not toxic for cells in culture. As a first try of vectorization we covered PNDs with plasmid DNA and examined the transfection efficiency. 196 pp. Englisch.…

Zustand: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Faklaris OrestisOrestis Faklaris, doctor in physical sciences, studies at University of Athens, master at Institut d Optique and PhD at ENS de Cachan, work on photoluminescent nanoparticles for cell labeling, super-resolution micros.…

Taschenbuch. Zustand: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This work studies the use of Photoluminescent NanoDiamonds (PNDs) for bio-imaging applications. The work is divided in two parts. The first part concerns the study of the optical properties of nitrogen-vacancy (NV) color centers and the optimization of the NV center concentration in nanodiamonds. To enhance the imaging contrast of internalized by cells PNDs, we applied time gated imaging and studied the 2-photon excitation properties of NV centers. While implementing a pulsed excitation laser, we discovered that simultaneous one and two-photon excitation quenches the signal of PNDs. We examined how this effect can serve for super-resolution imaging of NV centers. The second part of the work is devoted to the applications of PNDs as bio-imaging probes. In the prospect of applications of PNDs as drug delivery vehicles, we studied the uptake and elucidated the intracellular localization of PNDs by blocking different entry mechanisms and by immunofluorescence experiments. Moreover we ensured that PNDs are not toxic for cells in culture. As a first try of vectorization we covered PNDs with plasmid DNA and examined the transfection efficiency.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 196 pp. Englisch.…

Taschenbuch. Zustand: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - This work studies the use of Photoluminescent NanoDiamonds (PNDs) for bio-imaging applications. The work is divided in two parts. The first part concerns the study of the optical properties of nitrogen-vacancy (NV) color centers and the optimization of the NV center concentration in nanodiamonds. To enhance the imaging contrast of internalized by cells PNDs, we applied time gated imaging and studied the 2-photon excitation properties of NV centers. While implementing a pulsed excitation laser, we discovered that simultaneous one and two-photon excitation quenches the signal of PNDs. We examined how this effect can serve for super-resolution imaging of NV centers. The second part of the work is devoted to the applications of PNDs as bio-imaging probes. In the prospect of applications of PNDs as drug delivery vehicles, we studied the uptake and elucidated the intracellular localization of PNDs by blocking different entry mechanisms and by immunofluorescence experiments. Moreover we ensured that PNDs are not toxic for cells in culture. As a first try of vectorization we covered PNDs with plasmid DNA and examined the transfection efficiency.…