Bone research protocols (28 Ergebnisse)

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Taschenbuch. Zustand: Neu. Neuware - The last decade has seen a tremendous advance in our understanding of bone biology. The genes responsible for the majority of rare inherited bone disorders have been identified and much progress has been made in the identification of genes in polygenic disorders such as Paget's disease and complex multigene diseases such as osteoporosis. Transgenic technology has identified further genes, sometimes unexpectedly, with profound effects on bone. This wealth of new genetic information will undoubtedly lead to extensive cell biological studies to understand the mechanisms by which these gene products affect bone mass and bone strength. In Bone Research Protocols a catalogue of protocols has been assembled to perform such mechanistic studies. In the tradition of the Methods in Molecular Medicine series, the chapters are practical laboratory protocols that should enable the reader to carry out the techniques from scratch. To our knowledge this is the first time such a truly practical manual on well-established bone methods has been assembled, and this volume aims to be complementary to and follow on from the more theoretical Methods in Bone Biology, edited by Arnett and Henderson (1).…

Sprache: Englisch
Verlag: Springer, 2026
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Taschenbuch. Zustand: Neu. Bone Research Protocols | Aymen I. Idris | Taschenbuch | Methods in Molecular Biology | xvi | Englisch | 2026 | Springer | EAN 9781071643082 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu. …

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Sprache: Englisch
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Taschenbuch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - This third edition volume expands on the previous editions with new chapters and updated discussions on the latest advancements in the fields of musculoskeletal research and cancer-induced bone disease (CIBD). The chapters in this book are organized in to six parts and cover a wide range of established and new research procedures. Part One looks at methods for isolation, generation and analysis of osteoclasts, stem cells, circulating tumor cells, and bone marrow adipocytes. Part Two explores biochemical and molecular analysis procedures for isolation, purification, and quantification or mRNA and DNA in bone cells. Part Three focuses on ex vivo models of tissues, organs, and co-culture systems for bone and cancer cells, and Part Four presents various cancer related in vivo models of primary bone and secondary cancers in the skeleton. Part 5 discusses the frequently used bone microscopical and imaging analytical techniques, such as bone histomorphometry, immunostaining, and MicroCT scanning of bone. Finally Part Six talks about applications of GWAS, EWAS, systematic review, and meta-analysis. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble3.shooting and avoiding known pitfalls.Cutting-edge and comprehensive,Bone Research Protocols, Third Editionis a valuable resource for all researchers, scientists, and clinicians who are interested in learning more about this important and developing field.…

Sprache: Englisch
Verlag: Humana, 2025
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Zustand: New. 2025. Fourth Edition 2025. hardcover. . . . . .

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Sprache: Englisch
Verlag: Humana, 2025
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Zustand: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Contains a catalogue of protocols to assist researchers in the pursuit of mechanistic studiesProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the expertsStudies over.…

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Taschenbuch. Zustand: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Studies over the past decade have continued to bring tremendous advances to our understanding of bone biology. New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength. In Bone Research Protocols, Second Edition, expert researchers in the field detail many methods commonly used to study bone biology. Focusing mainly on in vitro methods, this volume gives techniques for isolation, culture and functional analysis of all bone cell types and details a range of imaging methods, including light and ultrastructural microscopy and live cell imaging. Some important in vivo techniques are included, such as analysis of bone resorption and imaging using X rays, fluorescent or luminescent techniques. Methods for study of proteins and nucleic acid are included and methods for analysis of bone composition, measurement of bone strength, and response to mechanical stimulation are described. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Bone Research Protocols, Second Edition seeks to aid scientists in the bone field to establish new techniques in their laboratories.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 660 pp. Englisch.…

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Taschenbuch. Zustand: Neu. This item is printed on demand - Print on Demand Titel. Neuware -The last decade has seen a tremendous advance in our understanding of bone biology. The genes responsible for the majority of rare inherited bone disorders have been identified and much progress has been made in the identification of genes in polygenic disorders such as Paget¿s disease and complex multigene diseases such as osteoporosis. Transgenic technology has identified further genes, sometimes unexpectedly, with profound effects on bone. This wealth of new genetic information will undoubtedly lead to extensive cell biological studies to understand the mechanisms by which these gene products affect bone mass and bone strength. In Bone Research Protocols a catalogue of protocols has been assembled to perform such mechanistic studies. In the tradition of the Methods in Molecular Medicine series, the chapters are practical laboratory protocols that should enable the reader to carry out the techniques from scratch. To our knowledge this is the first time such a truly practical manual on well-established bone methods has been assembled, and this volume aims to be complementary to and follow on from the more theoretical Methods in Bone Biology, edited by Arnett and Henderson (1).Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 464 pp. Englisch.…

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Taschenbuch. Zustand: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Studies over the past decade have continued to bring tremendous advances to our understanding of bone biology. New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength. In Bone Research Protocols, Second Edition, expert researchers in the field detail many methods commonly used to study bone biology. Focusing mainly on in vitro methods, this volume gives techniques for isolation, culture and functional analysis of all bone cell types and details a range of imaging methods, including light and ultrastructural microscopy and live cell imaging. Some important in vivo techniques are included, such as analysis of bone resorption and imaging using X rays, fluorescent or luminescent techniques. Methods for study of proteins and nucleic acid are included and methods for analysis of bone composition, measurement of bone strength, and response to mechanical stimulation are described. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bone Research Protocols, Second Edition seeks to aid scientists in the bone field to establish new techniques in their laboratories.…

Sprache: Englisch
Verlag: Springer Verlag Gmbh Jun 2026, 2026
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Taschenbuch. Zustand: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This third edition volume expands on the previous editions with new chapters and updated discussions on the latest advancements in the fields of musculoskeletal research and cancer-induced bone disease (CIBD). The chapters in this book are organized in to six parts and cover a wide range of established and new research procedures. Part One looks at methods for isolation, generation and analysis of osteoclasts, stem cells, circulating tumor cells, and bone marrow adipocytes. Part Two explores biochemical and molecular analysis procedures for isolation, purification, and quantification or mRNA and DNA in bone cells. Part Three focuses on ex vivo models of tissues, organs, and co-culture systems for bone and cancer cells, and Part Four presents various cancer related in vivo models of primary bone and secondary cancers in the skeleton. Part 5 discusses the frequently used bone microscopical and imaging analytical techniques, such as bone histomorphometry, immunostaining, and MicroCT scanning of bone. Finally Part Six talks about applications of GWAS, EWAS, systematic review, and meta-analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble3.shooting and avoiding known pitfalls.Cutting-edge and comprehensive, Bone Research Protocols, Third Edition is a valuable resource for all researchers, scientists, and clinicians who are interested in learning more about this important and developing field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 768 pp. Englisch.…

Sprache: Englisch
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This third edition volume expands on the previous editions with new chapters and updated discussions on the latest advancements in the fields of musculoskeletal research and cancer-induced bone disease (CIBD). The chapters in this book are organized in to six parts and cover a wide range of established and new research procedures. Part One looks at methods for isolation, generation and analysis of osteoclasts, stem cells, circulating tumor cells, and bone marrow adipocytes. Part Two explores biochemical and molecular analysis procedures for isolation, purification, and quantification or mRNA and DNA in bone cells. Part Three focuses on ex vivo models of tissues, organs, and co-culture systems for bone and cancer cells, and Part Four presents various cancer related in vivo models of primary bone and secondary cancers in the skeleton. Part 5 discusses the frequently used bone microscopical and imaging analytical techniques, such as bone histomorphometry, immunostaining, and MicroCT scanning of bone. Finally Part Six talks about applications of GWAS, EWAS, systematic review, and meta-analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble3.shooting and avoiding known pitfalls.Cutting-edge and comprehensive, Bone Research Protocols, Third Edition is a valuable resource for all researchers, scientists, and clinicians who are interested in learning more about this important and developing field. 768 pp. Englisch. …

Sprache: Englisch
Verlag: Humana Jun 2025, 2025
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Buch. Zustand: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This third edition volume expands on the previous editions with new chapters and updated discussions on the latest advancements in the fields of musculoskeletal research and cancer-induced bone disease (CIBD). The chapters in this book are organized in to six parts and cover a wide range of established and new research procedures. Part One looks at methods for isolation, generation and analysis of osteoclasts, stem cells, circulating tumor cells, and bone marrow adipocytes. Part Two explores biochemical and molecular analysis procedures for isolation, purification, and quantification or mRNA and DNA in bone cells. Part Three focuses on ex vivo models of tissues, organs, and co-culture systems for bone and cancer cells, and Part Four presents various cancer related in vivo models of primary bone and secondary cancers in the skeleton. Part 5 discusses the frequently used bone microscopical and imaging analytical techniques, such as bone histomorphometry, immunostaining, and MicroCT scanning of bone. Finally Part Six talks about applications of GWAS, EWAS, systematic review, and meta-analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble3.shooting and avoiding known pitfalls.Cutting-edge and comprehensive, Bone Research Protocols, Third Edition is a valuable resource for all researchers, scientists, and clinicians who are interested in learning more about this important and developing field.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 768 pp. Englisch.…

Sprache: Englisch
Verlag: Humana, 2025
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Buch. Zustand: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - This third edition volume expands on the previous editions with new chapters and updated discussions on the latest advancements in the fields of musculoskeletal research and cancer-induced bone disease (CIBD). The chapters in this book are organized in to six parts and cover a wide range of established and new research procedures. Part One looks at methods for isolation, generation and analysis of osteoclasts, stem cells, circulating tumor cells, and bone marrow adipocytes. Part Two explores biochemical and molecular analysis procedures for isolation, purification, and quantification or mRNA and DNA in bone cells. Part Three focuses on ex vivo models of tissues, organs, and co-culture systems for bone and cancer cells, and Part Four presents various cancer related in vivo models of primary bone and secondary cancers in the skeleton. Part 5 discusses the frequently used bone microscopical and imaging analytical techniques, such as bone histomorphometry, immunostaining, and MicroCT scanning of bone. Finally Part Six talks about applications of GWAS, EWAS, systematic review, and meta-analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble3.shooting and avoiding known pitfalls.Cutting-edge and comprehensive, Bone Research Protocols, Third Edition is a valuable resource for all researchers, scientists, and clinicians who are interested in learning more about this important and developing field.…