Gene isolation mapping protocols (44 Ergebnisse)

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Paperback. Zustand: Good. Over the past 10 years great progress has been made in the development of efficient techniques for both gene isolation and mapping. The identifica- tion and isolation of transcribed sequences from large chromosomal regions are central to the human genome mapping project. Techniques for isolating novel cDNAs have applications both in the overall construction and integra- tion of long-range physical and transcription maps and in the identification of disease genes. A number of different techniques for the isolation of cDNAs from mam- malian genomes have been developed, including screening zoo blots, the use of large genomic clones (YACs or cosmids) for hybridization against cDNA libraries, and CpG island mapping. More recently two highly efficient tech- niques have been introduced: exon trapping, based on the presence of exon splice sites, and direct selection, based on the enrichment of selected cDNAs using immobilized YACs or cosmids. Leading researchers in the field have contributed chapters detailing the practical procedures for these and other widely used methods. The most rapid progress presently being made in the field of gene isolation concerns the partial sequencing of cDNA clones from one or both ends to produce expressed sequence tags (ESTs). Indeed, by Octo- ber 1995, the EST division of Genbank (dbEST) contained a total of approxi- mately 270,000 human EST sequences accounting for almost half the number of sequence entries in Genbank. …

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Ringbuch. Zustand: Neu. 352 pp., numerous figs. and tabs. Neu! - Versandfertig am nächsten Tag; Rechnung mit MwSt. liegt bei! Gene Mapping Protocols is designed for newcomers wishing to learn the techniques applied by experts in gene mapping. Written with sufficient background material, and in a step-by-step format, it covers all the major techniques, including the localization. Sprache: Englisch.…

Zustand: Good. [ No Hassle 30 Day Returns ][ Ships Daily ] [ Underlining/Highlighting: NONE ] [ Writing: SOME ] [ Edition: First ] Slight markings on the cover Publisher: Humana Press Pub Date: 1/1/1996 Binding: Hardcover Pages: 328 First edition.

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Zustand: Sehr gut. Zustand: Sehr gut | Seiten: 332 | Sprache: Englisch | Produktart: Bücher | Over the past 10 years great progress has been made in the development of efficient techniques for both gene isolation and mapping. The identifica­ tion and isolation of transcribed sequences from large chromosomal regions are central to the human genome mapping project. Techniques for isolating novel cDNAs have applications both in the overall construction and integra­ tion of long-range physical and transcription maps and in the identification of disease genes. A number of different techniques for the isolation of cDNAs from mam­ malian genomes have been developed, including screening "zoo" blots, the use of large genomic clones (YACs or cosmids) for hybridization against cDNA libraries, and CpG island mapping. More recently two highly efficient tech­ niques have been introduced: exon trapping, based on the presence of exon splice sites, and direct selection, based on the enrichment of selected cDNAs using immobilized YACs or cosmids. Leading researchers in the field have contributed chapters detailing the practical procedures for these and other widely used methods. The most rapid progress presently being made in the field of gene isolation concerns the partial sequencing of cDNA clones from one or both ends to produce expressed sequence tags (ESTs). Indeed, by Octo­ ber 1995, the EST division of Genbank (dbEST) contained a total of approxi­ mately 270,000 human EST sequences accounting for almost half the number of sequence entries in Genbank.…

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Zustand: New. Designed for researchers wishing to learn the techniques applied by experts in gene mapping. The text covers the major techniques, including the localization of genes by FISH and linkage analysis, gene isolation by YAC hybridization, direct selection, and exon trapping and differential display. Series: Methods in Molecular Biology. Num Pages: 318 pages, biography. BIC Classification: PSAK; PSF. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 235 x 155 x 26. Weight in Grams: 721. . 1996. Hardback. . . . .…

Zustand: New. This text is designed for those wishing to learn the techniques applied by experts in gene mapping. It includes: the localization of genes by FISH and linkage analysis; gene isolation by YAC hybridization; direct selection; and exon trapping and differential display. Series: Methods in Molecular Biology. Num Pages: 318 pages, biography. BIC Classification: PSAK; PSF. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 229 x 152 x 18. Weight in Grams: 1080. . 1996. Paperback. . . . .…

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