Molecular biotechnology principles practices (10 Ergebnisse)

Soft cover. Zustand: New. Contents: Preface. I. Introduction to Biotechnology: 1. Biotechnology: scope and importance. 2. Biosafety and good laboratory practices. II. Advanced techniques in molecular biology: 3. Techniques of cell fractionation and centrifugation. 4. Chemical synthesis of nucleic acids. 5. DNA Chip Technology and its potential applications. 6. Bioinformatics in biotechnology. III. Working with nucleic acids: 7. Isolation of nucleic acids. 8. Measuring nucleic acid concentration and purity. 9. Electrophoretic techniques. 10. DNA sequencing. 11. Genetic maps and marker analysis. 12. Polymerase Chain Reaction (PCR). 13. In Situ hybridization. IV. Recombinant DNA and genetic engineering: 14. Fundamentals of recombinant DNA technology. 15. Enzymes in molecular cloning. 16. Gene constructs and cloning vectors. 17. DNA libraries. 18. Molecular biology of gene transfer systems. 19. Selection and screening of recombinant molecules. V. Applications of biotechnology: 20. Genetic engineering of microorganisms. 21. Genetic engineering of animals. 22. Genetic engineering in plants. VI. Working with proteins: 23. Protein purification techniques. 24. Protein detection and estimation. 25. Protein fractionation techniques. 26. Immunochemical techniques. VII. Bacterial and mammalian cell culture: 27. Biology of bacteria. 28. Cultivation of mammalian cells In vitro. VIII. In Vitro plant cell culture and crop improvement: 29. Plant cell culture laboratory and requirements. 30. Plant culture media, preparation and culture initiation. 31. Micropropagation. 32. Cultures of organized tissues. 33. Culture of unorganized tissues. 34. Cryopreservation and distribution of clonal material. 35. Measurement of plant cell growth and cytological analysis. 36. Protoplast fusion and Somaclonal variation. 37. Application of plant cell, tissue and organ culture. IX. Environmental biotechnology: 38. Biotechnology in pollution control. 39. Biodiversity and genetic conservation. 40. Bioenergy fuel from biomass. 41. Regulatory aspects of using genetically-modified organisms. 42. Intellectual property rights and socio-legal aspects of biotechnology. Appendices. Index. "In the recent past, biotechnology has emerged as an important tool especially in the economic sectors of agriculture, livestock management, human health care and pharmaceutical industries, and for solving environmental and societal issues. As a result, many universities now offer various programmes in biotechnology and related fields. Molecular Biotechnology : Principles and Practices is intended as a student and teacher friendly textbook aimed at providing undergraduate and postgraduate students with a strong base in this emerging and highly promising interdisciplinary science. It strikes a balance between two important aspects of the science--the theory of molecular biology and the experimental approach to the study of biological processes. The main feature of this book is its coverage of a wide range of molecular techniques in biotechnology. Each technique is described conceptually, followed by a detailed experimental account of the steps involved. The topics covered include: Scope of biotechnology. Advanced techniques used in molecular biology and genetics. Gene cloning technology and its applications. Genetic engineering and how it revolutionized biotechnology. Commercial potential of tissue culture technology. Social, economic, and legal issues.".…

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