Protein lipidation protocols (33 Ergebnisse)

gebundene Ausgabe. Zustand: Gut. 240 Seiten; Das hier angebotene Buch stammt aus einer teilaufgelösten wissenschaftlichen Bibliothek und trägt die entsprechenden Kennzeichnungen (Rückenschild, Instituts-Stempel.); Schnitt und Einband sind etwas staubschmutzig; der Buchzustand ist ansonsten ordentlich und dem Alter entsprechend gut. Das Buch ist auf ENGLISCH. Sprache: Englisch Gewicht in Gramm: 550.…

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Taschenbuch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume explores techniques used to detect lipids attached to proteins, to analyze the function of lipid modifications, and to characterize the enzymes that add and remove lipids from proteins. The book is organized into seven parts: Part One describes chemically-based strategies to identify substrates for protein lipidation that can be applied to individual proteins or globally using proteomics. Part Two focuses on the enzymes that remove fatty acids from proteins and provides methods to monitor protein biogenesis and palmitate turnover. Part Three addresses biochemical and cellular characterization of DHHC S-acyltransferases, a family of enzymes with 23 members encoded by the human genome. Part Four presents the SwissPalm 2 database and tips on how to use it effectively. Part Five focuses on fatty acylation that occurs in the lumen of the secretory pathway. Parts Six and Seven conclude the book with methods to produce and assay lipid-modified and integral membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Protein Lipidation: Methods and Protocols is a valuable resource for experts in the field and for investigators who encounter protein lipidation through their research on a particular cellular process or favorite protein.…

Taschenbuch. Zustand: Neu. Protein Lipidation | Methods and Protocols | Maurine E. Linder | Taschenbuch | xiv | Englisch | 2020 | Humana | EAN 9781493995349 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu.…

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Zustand: New. Editor(s): Gelb, Michael H. Series: Methods in Molecular Biology. Num Pages: 240 pages, 35 black & white illustrations, biography. BIC Classification: PSB. Category: (P) Professional & Vocational. Dimension: 229 x 152 x 13. Weight in Grams: 374. . 2013. Softcover reprint of the original 1st ed. 1999. Paperback. . . . .…

Zustand: New. pp. 341.

Zustand: New. This text contains a collection of readily reproducible techniques for studying protein lipidation, the covalent attachment of lipids to proteins. These cutting-edge methods deal with glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. Series: Methods in Molecular Biology. Num Pages: 240 pages, 35 black & white illustrations, biography. BIC Classification: PSBC; PSBH. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 235 x 155 x 15. Weight in Grams: 537. . 1999. Hardback. . . . .…

Taschenbuch. Zustand: Neu. Protein Lipidation Protocols | Michael H. Gelb | Taschenbuch | x | Englisch | 2013 | Humana | EAN 9781489943309 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu. …

Zustand: New. pp. 252.

Gebunden. Zustand: New. In Vitro Analysis of GPI Biosynthesis in Mammalian Cells, Victoria L. Stevens. Selection of Mammalian Cell Mutants in GPI Biosynthesis, Victoria L. Stevens. Analysis of the Cell-Surface Distribution of GPI-Anchored Proteins, Satyajit Mayor. Imaging Fluoresc.

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Zustand: New. Editor(s): Gelb, Michael H. Series: Methods in Molecular Biology. Num Pages: 240 pages, 35 black & white illustrations, biography. BIC Classification: PSB. Category: (P) Professional & Vocational. Dimension: 229 x 152 x 13. Weight in Grams: 374. . 2013. Softcover reprint of the original 1st ed. 1999. Paperback. . . . . Books ship from the US and Ireland.…

Zustand: New. This text contains a collection of readily reproducible techniques for studying protein lipidation, the covalent attachment of lipids to proteins. These cutting-edge methods deal with glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. Series: Methods in Molecular Biology. Num Pages: 240 pages, 35 black & white illustrations, biography. BIC Classification: PSBC; PSBH. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 235 x 155 x 15. Weight in Grams: 537. . 1999. Hardback. . . . . Books ship from the US and Ireland.…

Buch. Zustand: Neu. Neuware - In Protein Lipidation Protocols, Michael Gelb brings together a collection of readily reproducible techniques for studying protein lipidation, the covalent attachment of lipids to proteins. These cutting-edge methods-many never published before in a 'hands-on' format-deal with glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. Included are novel techniques for determining the chemical structure of GPI-anchors, for radiolabeling the prenyl groups of protein in eukaryotic cells, a tool for developing inhibitors of the protein farnesyltransferase, and for an exciting lysosomal enzyme that cleaves fatty acyl groups from proteins, the first fatty acylase discovered. Protein Lipidation Protocols offers biochemists, cell and molecular biologists, medicinal chemists, and pharmaceutical researchers state-of-the-art tools for understanding the complex biochemistry of protein lipidation, as well as catalyzing the development of many important new biopharmaceuticals, including anticancer drugs. …

Zustand: New. 1st ed. 2019 edition NO-PA16APR2015-KAP.

Hardcover. Zustand: Brand New. 327 pages. 10.00x7.25x1.00 inches. In Stock.

Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In Protein Lipidation Protocols, Michael Gelb brings together a collection of readily reproducible techniques for studying protein lipidation, the covalent attachment of lipids to proteins. These cutting-edge methods-many never published before in a 'hands-on' format-deal with glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. Included are novel techniques for determining the chemical structure of GPI-anchors, for radiolabeling the prenyl groups of protein in eukaryotic cells, a tool for developing inhibitors of the protein farnesyltransferase, and for an exciting lysosomal enzyme that cleaves fatty acyl groups from proteins, the first fatty acylase discovered. Protein Lipidation Protocols offers biochemists, cell and molecular biologists, medicinal chemists, and pharmaceutical researchers state-of-the-art tools for understanding the complex biochemistry of protein lipidation, as well as catalyzing the development of many important new biopharmaceuticals, including anticancer drugs. 252 pp. Englisch.…

Zustand: new. Questo è un articolo print on demand.

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Zustand: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. In Vitro Analysis of GPI Biosynthesis in Mammalian Cells, Victoria L. Stevens. Selection of Mammalian Cell Mutants in GPI Biosynthesis, Victoria L. Stevens. Analysis of the Cell-Surface Distribution of GPI-Anchored Proteins, Satyajit Mayor. Imaging Fluoresc.…

Taschenbuch. Zustand: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - In Protein Lipidation Protocols, Michael Gelb brings together a collection of readily reproducible techniques for studying protein lipidation, the covalent attachment of lipids to proteins. These cutting-edge methods-many never published before in a 'hands-on' format-deal with glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. Included are novel techniques for determining the chemical structure of GPI-anchors, for radiolabeling the prenyl groups of protein in eukaryotic cells, a tool for developing inhibitors of the protein farnesyltransferase, and for an exciting lysosomal enzyme that cleaves fatty acyl groups from proteins, the first fatty acylase discovered. Protein Lipidation Protocols offers biochemists, cell and molecular biologists, medicinal chemists, and pharmaceutical researchers state-of-the-art tools for understanding the complex biochemistry of protein lipidation, as well as catalyzing the development of many important new biopharmaceuticals, including anticancer drugs.…

Taschenbuch. Zustand: Neu. This item is printed on demand - Print on Demand Titel. Neuware -In Vitro Analysis of GPI Biosynthesis in Mammalian Cells.- Selection of Mammalian Cell Mutants in GPI Biosynthesis.- Analysis of the Cell-Surface Distribution of GPI-Anchored Proteins.- Imaging Fluorescence Resonance Energy Transfer as Probe of Membrane Organization and Molecular Associations of GPI-Anchored Proteins.- Purification of Caveolae-Derived Membrane Microdomains Containing Lipid-Anchored Signaling Molecules, Such as GPI-Anchored Proteins, H-Ras, Src-Family Tyrosine Kinases, eNOS, and G-Protein -, -, and -Subunits.- Analysis of Lipids in Caveolae.- Analysis of the Carbohydrate Components of Glycosylphosphatidylinositol Structures Using Fluorescent Labeling.- Analysis of the Lipid Moiety of GPI Anchor in the Yeast Saccharomyces cerevisiae.- Rapid Identification of Cysteine-Linked Isoprenyl Groups by Metabolic Labeling with [3H]Farnesol and [3H]Geranylgeraniol.- Incorporation of Radiolabeled Prenyl Alcohols and Their Analogs into Mammalian Cell Proteins.- Reconstitution of Yeast Farnesyltransferase from Individually Purified Subunits.- Probing the Role of H-Ras Lipidation for Signaling Functions in Xenopus laevis Oocytes.- Fluorescence Measurement of Lipid-Binding Affinity and Interbilayer Transfer of Bimane-Labeled Lipidated Peptides.- Determination of the Kinetics of Intervesicle Transfer and Transbilayer Diffusion of Bimane-Labeled Lipidated Peptides.- Preparation and Assay of Myristoyl-CoA:Protein N-Myristoyltransferase.- Metabolic Labeling of Protein-Derived Lipid Thioesters in Palmitoyl-Protein Thioesterase-Deficient Cells.- Fatty Acid Analysis of Protein-Derived Lipid Thioesters Isolated from Palmitoyl-Protein Thioesterase-Deficient Cells.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 252 pp. Englisch.…

Zustand: New. Print on Demand pp. 341.

Zustand: New. PRINT ON DEMAND pp. 341.

Zustand: New. Print on Demand pp. 252 23:B&W 6 x 9 in or 229 x 152 mm Perfect Bound on White w/Gloss Lam.

Zustand: New. PRINT ON DEMAND pp. 252.