Retinal gene therapy methods (27 Ergebnisse)

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Taschenbuch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - This detailed volume describes a spectrum of methods and protocols that can be used for the bench-to-bedside development and evaluation of retinal gene therapy. Methods for the successful delivery of these gene therapy vector systems to the retina are examined, as well as assays to test the efficacy in vitro in cell cultures, for gene augmentation and gene editing in vivo on rodents, pigs, and monkey retinas, and on human retinal explants as well as in human clinical studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Retinal Gene Therapy: Methods and Protocols provides a wide range of readers from students to research experts with vital information on ocular gene therapy vector technology, in vitro and in vivo biological assays, and clinical protocols, to promote further studies for the benefit of children and adults with inherited retinal disease.…

Taschenbuch. Zustand: Neu. Retinal Gene Therapy | Methods and Protocols | Camiel J. F. Boon (u. a.) | Taschenbuch | Methods in Molecular Biology | xvi | Englisch | 2018 | Springer | EAN 9781493985098 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu.…

Zustand: New. Softcover reprint of the original 1st ed. 2018 edition NO-PA16APR2015-KAP.

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Zustand: New. In English.

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Buch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - This detailed volume describes a spectrum of methods and protocols that can be used for the bench-to-bedside development and evaluation of retinal gene therapy. Methods for the successful delivery of these gene therapy vector systems to the retina are examined, as well as assays to test the efficacy in vitro in cell cultures, for gene augmentation and gene editing in vivo on rodents, pigs, and monkey retinas, and on human retinal explants as well as in human clinical studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Retinal Gene Therapy: Methods and Protocols provides a wide range of readers from students to research experts with vital information on ocular gene therapy vector technology, in vitro and in vivo biological assays, and clinical protocols, to promote further studies for the benefit of children and adults with inherited retinal disease.…

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Buch. Zustand: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - This volume covers the latest advancements in the study of retinal gene therapy and addresses critical aspects of the gene therapy development pipeline. The chapters in this book are organized into four parts. Part One focuses on vector development and design, and talks about the latest approaches used to enhance the specificity and efficiency of gene delivery to retinal cells. Part Two presents a series of in vitro assays that are important for validating retinal gene therapies. Part Three discusses in vivo proof-of-concept studies and therapy validation in animal models. Part Four addresses clinical validation and presents methods for assessing visual function in human subjects undergoing retinal gene therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Retinal Gene Therapy: Methods and Protocols is a valuable resource for researchers, clinicians, and students who are interested in learning more about this rapidly evolving field and looking for new research directions and collaborations across disciplines.…

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