Rna imaging methods protocols (23 Ergebnisse)

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Taschenbuch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - This detailed volume presents a comprehensive guide to live-cell RNA imaging techniques and methodologies that have been successfully used to study the dynamics or function of various RNAs in cells. The topics covered encompass techniques including the detection of aptamer-tagged mRNA molecules utilizing fluorogenic proteins, monitoring endogenous mRNA via a split-protein-based probe within neuronal cells, sensitive detection of low-abundance RNAs employing catalytic hairpin assembly circuits, monitoring RNA dynamics using the MS2 system within plant cells, detection of miRNA utilizing DNA-MOF probes, and identification of RNA G-quadruplex structures or nucleolar RNA through fluorescent probes, among other advanced techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Live-Cell RNA Imaging: Methods and Protocols provides researchers with a diverse toolkit for investigating RNA dynamics and function within live cells, thereby contributing to advancements in our understanding of cellular processes at the molecular level.…

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Taschenbuch. Zustand: Neu. Live-Cell RNA Imaging | Methods and Protocols | Shin-Ichi Sato | Taschenbuch | Methods in Molecular Biology | xi | Englisch | 2025 | Springer | EAN 9781071642504 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu. …

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Buch. Zustand: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - This detailed volume will focus on the phenomenon of RNA interference by providing comprehensive coverage of various techniques for in vivo micro/siRNA imaging including the design and synthesis of specific imaging agents and tools, the development of imaging methodologies, and their interpretation. An essential element in the development and optimization of these therapies is the ability to measure the bioavailability and functionality of the RNA/oligonucleotide molecule after administration into the body. Noninvasive imaging provides the necessary set of tools to accomplish this in authentic physiologic environments and across time. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, RNA Imaging: Methods and Protocols serves physicians, scientists, and graduate students who are either new to the field of RNA-based imaging and its associated therapeutic applications or who wish to be apprised of recent advances in the state of the art.…

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Buch. Zustand: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - This detailed volume presents a comprehensive guide to live-cell RNA imaging techniques and methodologies that have been successfully used to study the dynamics or function of various RNAs in cells. The topics covered encompass techniques including the detection of aptamer-tagged mRNA molecules utilizing fluorogenic proteins, monitoring endogenous mRNA via a split-protein-based probe within neuronal cells, sensitive detection of low-abundance RNAs employing catalytic hairpin assembly circuits, monitoring RNA dynamics using the MS2 system within plant cells, detection of miRNA utilizing DNA-MOF probes, and identification of RNA G-quadruplex structures or nucleolar RNA through fluorescent probes, among other advanced techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Live-Cell RNA Imaging: Methods and Protocols provides researchers with a diverse toolkit for investigating RNA dynamics and function within live cells, thereby contributing to advancements in our understanding of cellular processes at the molecular level.…