humana press humana press aug 2016 (Mehr als 1.300 Ergebnisse)

Taschenbuch. Zustand: Neu. Neuware -Providing experimental methods and protocols for performing pheromone research in a variety of organisms ranging from invertebrates to vertebrates, Pheromone Signaling: Methods and Protocols covers a wide spectrum of experimental approaches necessary for handling pheromone molecules, measuring receptor response and neural activation, and analyzing behavioral output. A great deal of progress has been made in understanding the molecular mechanisms underlying pheromone action, largely due to the discovery of receptor genes, the advancement of imaging techniques, and key multi-disciplinary approaches including aspects of organic chemistry, biochemistry, molecular biology, electrophysiology, and behavioral science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Pheromone Signaling: Methods and Protocols details the key methodologies utilized in laboratories all over the world, making them accessible to those who want to begin investigation in the area of pheromone research. 416 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Nanopores are vital biological features, described as tiny holes in cellular membranes used for recognition and transport of ions and molecules between compartments within the cell, as well as between the extracellular environment and the cell itself. Their study, ever growing in esteem, leads toward the promise of ultra-fast sequencing of DNA molecules with the ultimate goal of building a nanoscale device that will make rapid and cheap DNA sequencing a reality. In Nanopore-Based Technology, expert researchers in the forefront of the field explore the cutting-edge of nanopore technology for single molecule sensing, detection, and characterization. Divided into four convenient parts, this volume covers single molecule characterization techniques utilizing biological pores, methods for biomolecule characterization with nanoporous artificial membranes, computational studies of the biomolecule confined within the nanopore environment, as well as techniques that use novel materials in conjunction with nanopore sensing. Written for the highly successful Methods in Molecular Biology¿ series, this work provides the kind of detailed description and implementation advice that is crucial for achieving optimal results.Authoritative and state-of-the-art, Nanopore-Based Technology serves as an excellent representation of the present-day available techniques for biomolecule characterization with nanoporous membranes in order to guide researchers toward developing the next generation of technologies for fast and cheap DNA sequencing with practically no limitations on the read lengths.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 280 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Diagnosing and managing type 2 diabetes presents an enormous challenge to the primary care provider confronted with multiple emerging scientific insights, therapeutic strategies and risk reduction principles. In Type 2 Diabetes, Pre-Diabetes, and the Metabolic Syndrome: The Primary Care Guide to Diagnosis and Management, Second Edition, Ronald A. Codario, M.D., FACP -- a well-known and highly respected authority on diabetes -- details the state-of-the-art in diagnosing, managing and attenuating risks in patients with this disease.Utilizing his extensive experience in private practice, medical education and clinical research for over 35 years, Dr. Codario explains in simple clinical terms, the current understanding of the pathophysiology of diabetes, the latest clinical trials, developing controversies, updates on new medications and an expanded section on Special Populations. With his unique, multiple board certifications in clinical hypertension, vascular medicine, internal medicine, vascular ultrasound and clinical lipidology, Dr. Codario provides practical guidelines for treatment with insulin and oral agents, lipid and hypertension control and comprehensive risk reduction strategies.Extensively reviewed are the metabolic syndrome, the role of exercise and nutrition, and key issues associated with herb and nutriceutical use. Illustrative case studies in diabetes management, an outstanding bibliography of suggested readings, and extensive chapter subheadings for quick reference make this book a practical, easy-to-read guide for dealing with this killer disease.Type 2 Diabetes, Pre-Diabetes, and the Metabolic Syndrome: The Primary Care Guide to Diagnosis and Management, Second Edition is the direct result of many years of listening, teaching, lecturing and empathizing with fellow primary care providers and their patients in the ongoing fight against diabetes. Like the internationally acclaimed first edition, this is a must read and invaluable guide for allprimary care providers, students, caregivers and patients battling the ravages of this ever increasing epidemic.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 384 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Protein misfolding is a key feature of many disorders in humans, given that over twenty proteins are known to misfold and cause disease. In Protein Folding, Misfolding, and Disease: Methods and Protocols, experts in the field present a collection of current methods for studying the analysis of protein folding and misfolding, featuring strategies for expressing and refolding recombinant proteins which can then be utilized in subsequent experiments. This detailed volume also covers methods for analyzing the formation of amyloid, protocols for determining the size and structure of native and misfolded proteins, as well as specific examples of where misfolded proteins can be examined using state-of ¿the-art technologies. Written in the highly successful Methods in Molecular Biology¿ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Up to date and authoritative, Protein Folding, Misfolding, and Disease: Methods and Protocols offers researchers the tools necessary to move ahead in this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 260 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -In recent years, there have been many exciting breakthroughs in the application of nanotechnology to medicine. In Characterization of Nanoparticles Intended for Drug Delivery, expert researchers explore the latest advances in the field, providing a set of basic methods for the characterization of nanomaterials for medical use. Chapters provide methods to characterize the physiochemical properties (size, aggregation, and surface chemistry) and in vitro immunological and biological characteristics of nanomaterials. Composed in the highly successful Methods in Molecular Biology¿ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and informative, Characterization of Nanoparticles Intended for Drug Delivery is an essential survey of methods that are crucial to the preclinical characterization of nanomedicines.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 284 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Since the publication of the first edition, lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, continue to offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. Lentivirus Gene Engineering Protocols, Second Edition reflects the spectacular progress made in the field with a set of cutting-edge methods contributed by highly respected scientists. Beginning with a thorough overview of the most recent lentivirus developments, the book continues with detailed protocols including sections on the advances in lentiviral vector technology, new lentiviral vector applications, involving transgenic human embryonic stem cells and fetal gene therapy among other topics, as well as the invaluable breakthroughs in LV-mediated expression of microRNAs. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.Authoritative and timely, Lentivirus Gene Engineering Protocols, Second Edition covers the most relevant issues and techniques of LV-based gene engineering, thus representing a complete theoretical and practical guide for scientists still unfamiliar with LV technologies and those who simply wish to know more about this vital area of study.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 240 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Spanning from discoveries in fundamental immunology to industrial and commercial concerns, the study of vaccine adjuvants has developed into an exciting area of work with great, vital potential in innovating techniques in which adjuvants may steer the immune system towards the responses required by unmet vaccination needs. In Vaccine Adjuvants: Methods and Protocols, expert researchers in the field provide clear and concise guidance on how to go about assessing the activity of adjuvant products. Rather than describing individual adjuvants, the volume strives to include detailed, practical information on measuring the responses produced by adjuvants in order to be relevant to the widest array of experiments. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and versatile, Vaccine Adjuvants: Methods and Protocols will enable those already pursuing vaccine adjuvant research, while also serving to stimulate discussion on how to best standardize adjuvant testing in order to facilitate meaningful comparisons, and above all, to aid in the prediction of which new products will most effectively and safely help to solve the current challenges in vaccination.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 324 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques, all of which have been producing fundamental insights into the functions of cells and tissues. Chapters cover a variety of bright field and fluorescence microscopy-based approaches that are central to the study of a range of biological questions, providing information on how to prepare cells and tissues for microscopic investigations, covering detailed staining procedures, and exploring methods to analyze images and interpret the results accurately. Composed in the highly successful Methods in Molecular Biology¿ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and current, Light Microscopy: Methods and Protocols is an essential handbook for all researchers who are exploring the intriguing microscopic world of the cell.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 256 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -The next revolution in molecular medicine is the application of molecular profiling to individualized patient therapy. Molecular profiling technology has advanced dramatically, particularly in the field of cancer tissue biomarkers. It is now possible to gather complex genomic and proteomic information from a routine clinical needle biopsy or surgical specimen. In Molecular Profiling : Methods and Protocols, expert researchers in the field focus on the entire process from discovery to commercialization, with practical guides that are not limited to experimental methods. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as essays and guidelines for grants, patents, and commercialization of products related to molecular profiling.Authoritative and practical, Molecular Profiling: Methods and Protocols seeks to aid scientists in understanding the latest advancements in genomics, proteomics, imagining, and bioinformatics.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 464 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function. Written in the highly successful Methods in Molecular Biology¿ series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.Comprehensive and up-to-date, Chromatin Protocols, Second Edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 436 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -In Synthetic Biology, expert researchers in the field provide the latest developments in molecular biology techniques used in Synthetic Biology. Focusing on computational tools that will aid in systematising the design and construction of parts and systems. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Synthetic Biology seeks to aid scientists in the further study of developing new biological components and systems.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 236 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Urological Emergencies: A Practical Approach, Second Edition provides a concise, evidence based review of common urgent and emergent urological conditions in children and adults. Descriptions of the appropriate diagnostic and therapeutic considerations on topics of traumatic, infectious, obstructive, hemorrhagic, iatrogenic, vascular and congenital urological emergencies in adults and children are provided.The text is organized by pathophysiology rather than organ system, allowing the reader to develop approaches to the care of patients with acute urological conditions based on mechanism of disease. Relevant pathophysiological background and epidemiology are reviewed, necessary diagnostic testing recommended, and detailed medical, surgical and endourological management approaches have been provided. All chapters incorporate published guidelines and best practice recommendations from appropriate organizations including the American Urological Association, the Centers for Disease Control and Prevention, and various International Consultations on Urological Diseases. New or significantly revised chapters in each of the subsections address innovations in trauma care and prevention, new strategies in management of kidney stones, and special care needs of children with acute urological problems.Urological Emergencies: A Practical Approach, Second Edition is of great value to practicing urologists, urology residents, emergency medicine trainees and practitioners, and primary care providers including MD¿s, PA¿s, and NP¿s without immediate access to urological consultation.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 356 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Liquid-Chromatography-Mass-Spectrometry procedures have been shown to be successful when applied to drug development and analysis. LC-MS in Drug Analysis: Methods and Protocols provides detailed LC-MS/MS procedures for the analysis of several compounds of clinical significance. The first chapters provide the reader with an overview of mass spectroscopy, its place in clinical practice, its application of MS to TDM and toxicology, and the merits of LC-MS(/MS) and new sample preparation techniques. The following chapters discuss different approaches to screening for drugs of abuse and for general unknowns, as well as targeted measurement of specific analytes or classes of analytes including abused drugs, toxic compounds, and therapeutic agents. Written in the successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, LC-MS in Drug Analysis: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 236 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -In Functional Analysis of DNA and Chromatin, expert researchers in the field provide an overview of standard and more recent methods for the functional analysis of the genetic material. These include methods on DNA-dye binding modes, chromatin staining, nuclear dispersion assays, DNA labeling in vivo, sister chromatid exchanges, FISH, DNA and chromatin imaging by fluorescence, electron and atomic force microscopy, detection of apoptotic DNA, cytosine methylation and hydroxy-methylation, DNA thermophoresis, improved methods for histone analysis, chromatin immunoprecipitation, and analysis of rDNA genes and chromatin-associated RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritativeand practical,Functional Analysis of DNA and Chromatin seeks to aid scientists in the further study of cellular and molecular biology of the nucleus.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 384 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Plant Chemical Genomics: Methods and Protocols , expert researchers in the field detail many of the techniques used for identification of bioactive compounds from a large selection of fields in plant biology including plant pathogenesis, immune responses, small RNA processing, endomembrane trafficking, lipids, plant hormone signaling and cell wall. The presentation of these and other examples as well as synthetic chemistry, computation and target identification provides a comprehensive overview of the practical aspects of chemical biology that are possible in plant systems. Small molecules and natural products that produce a phenotype in plants have been known for decades. The agrichemical industry has utilized small molecules based on known natural molecules such as auxins, cytokinins, abscissic acid and other growth regulators. In recent years, the screening and characterization of novel small molecules has enhanced our understanding of protein function, metabolic, signaling and endomembrane pathways, and their interactions in basic plant research.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 284 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Many fundamental discoveries concerning epigenetics and the elucidation of mechanisms of epigenetic regulation have developed from studies performed in plants. In Plant Epigenetics and Epigenomics: Methods and Protocols, leading scientists in the epigenetics field describe comprehensive techniques that have been developed to understand the plant epigenetic landscape.These include recently developed methods and techniques for analysis of epigenetically regulated traits, such as flowering time, transposon activation, genomic imprinting and genome dosage effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoidance of known pitfalls.Authoritative and practical, Plant Epigenetics and Epigenomics: Methods and Protocols seek to aid scientists in the further study of plant epigenetic phenomena using advanced contemporary methods.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 256 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; however, human studies of its function are often limited by ethical, logistical, and technical obstacles. In Mouse Models of Innate Immunity: Methods and Protocols, experts in the field explore the design and execution of experiments used to thoroughly evaluate critical elements associated with the host innate immune response. The volume opens with methods that are essential for collecting and assessing various primary cells that are highly relevant to innate immunity, and it continues with in vivo protocols commonly used to evaluate the innate immune response in the mouse, including mouse models of respiratory infection, gastrointestinal inflammation, fungal and parasitic diseases, sepsis, and HIV-1 infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune response.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 240 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Exemplifying and illustrating recent exciting advances in PNA chemistry, the second edition of Peptide Nucleic Acids: Methods and Protocols serves as a vital complement to the first edition of the book. Since the discovery of peptide nucleic acids, many interesting new derivatives and analogues in terms of nucleic acid recognition specificity and affinity have emerged. Also, as this detailed volume presents, great ingenuity in exploiting the unique properties of PNAs for a wide variety of applications within drug discovery, medical diagnostics, chemical biology and nanotechnology has unfolded. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Peptide Nucleic Acids: Methods and Protocols, Second Editionserves as a source of useful specific methods and protocols as well as a source of inspiration for future developments.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 252 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -The discovery of stem and progenitor cells in the adult mammalian CNS challenged the long standing ¿no new neuron¿ doctrine and opened the door to the potential for cell replacement therapy. The process from discovery to clinical applications can be long and tortuous, requiring rigorous steps involving standardized and precise protocols. Neural Progenitor Cells: Methods and Protocols is a collection of practical articles describing techniques used to study neural stem and progenitor cells. The volume also highlights the promise of stem cell-based therapeutic applications for CNS disorders. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Neural Progenitor Cells: Methods and Protocols serves both professionals and novices with its well-honed methodologies.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 256 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Plant Cell Morphogenesis: Methods and Protocols provides a collection of experimental techniques used in current research on the cellular aspects of plant morphogenesis.Methods and techniques include contemporary takes on classical light microscopy and histochemistry through automated microscopy applications, use of advanced optical tools, quantitative image analysis, study of cellular dynamics of apical meristems, specialized electron microscopy techniques, and methods used to study specific model plant cell types and protocols for using heterologous expression in yeast to study cell morphogenesis genes. Individual chapters in the highly successful Methods in Molecular Biology series format are written by expert researchers in the field and include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 300 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -¿In Cereal Genomics: Methods and Protocols, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes. Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described. Tools for the isolation, characterization and functional analysis of cereal genes and their transcripts are detailed. Methods for molecular screening of cereals and for their genetic transformation are also covered.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Cereal Genomics: Methods and Protocols provides a comprehensive resource for those studying cereal genomes.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 312 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -In Peptide Modifications to Increase Metabolic Stability and Activity, expert researchers in the field provide summarized methods for preparation, purification of modified peptides, and assessment of their biochemical activities. These methods and protocols include preparation of conformationally constrained peptides, modification of peptide bonds, introduction of nonproteinogenic amino acids, and alteration of peptides¿ physical and biological properties by modification of the amino acid side chains and/or terminal residues. With additional chapter that describes new experimental approach for the detection of exogenous peptides within living cells using peptides labeled with heavy isotopes and confocal Raman microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Peptide Modifications to Increase Metabolic Stability and Activity seeks to provide scientists with alternative approaches to peptide modification that many researchers may find applicable to their specific research requirements.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 252 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -In Fluorescent Protein-Based Biosensors: Methods and Protocols, experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction. Genetically encodable fluorescent biosensors have allowed researchers to observe biochemical processes within the endogenous cellular environment with unprecedented spatiotemporal resolution. As the number and diversity of available biosensors grows, it is increasingly important to equip researchers with an understanding of the key concepts underlying the design and application of genetically encodable fluorescent biosensors to live cell imaging. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Fluorescent Protein-Based Biosensors: Methods and Protocols promises to be a valuable resource for researchers interested in applying current biosensors to the study of biochemical processes in living cells as well as those interested in developing novel biosensors to visualize other cellular phenomena.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 264 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 296 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -In Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols, expert researchers in the field provide a complete overview of protocols used in the development of RNA molecule as drugs and drug target. These include methods and protocols on recent and precise RNA molecule approaches or studies in the development of an RNA therapeutic tool. We are convinced that these methods will help researchers from various domains of life sciences, including clinicians, biochemists and virologists. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols seeks to aid scientists in the further development of new therapeutic applications implicating RNA molecules or directly targeting harmful RNA.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 292 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Therapeutic Peptides: Methods and Protocols features biological methods for the preparation of peptide phage display libraries using both filamentous and lytic phage. With contributions from renowned authors in the field, the book also explores selection and screening of the prepared peptide libraries for peptides with the desired function and the subsequent characterization of the identified peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and dependable, Therapeutic Peptides: Methods and Protocols is an ideal guide for researchers from all backgrounds seeking methods for the identification of therapeutic peptide candidates.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 292 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades. The chapters in Staphylococcus Epidermidis: Methods and Protocols are designed to give the new investigator a series of tools so they can ask novel and exciting questions related to the biology of this opportunistic pathogen, as many exciting and unexplored questions such as defining the interaction of S. epidermidis and other normal flora remain to be discovered. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Staphylococcus Epidermidis: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 220 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -In DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 312 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -In Tiling Arrays: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study tiling microarrays in genomic discovery . These include methods and applications for transcriptional regulation, expression, genetic and epigenetic regulation, as well as techniques and skills on tiling microarray data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Tiling Arrays: Methods and Protocols seeks to provide scientists with a comprehensive and down to earth approach to maximize results.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 248 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Myc controls multiple cellular functions, including cell proliferation, growth, differentiation and death, both directly and indirectly, through its modulation of downstream transcriptional programs. In The Myc Gene: Methods and Protocols, experts in the field summarize the standard and novel techniques that allow the studying of Myc mechanism of action in normal and cancer cells, in vitro and in vivo, in one succinct manual. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 296 pp. Englisch.