Springer us apr 2021 (24 Ergebnisse)
Sprache: Englisch
Verlag: Springer US Apr 2021 2021
Serie: Physiology in Health and Disease, Buch 8 von 13. Buch 8 von 13 - Physiology in Health and Disease
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EUR 106,99
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book highlights the importance of the choroid plexus, which forms the blood-cerebrospinal fluid barrier and is the site of the major production of the cerebrospinal fluid (CSF). The authors show that this barrier is crucial for m…aintaining important compositional differences between the blood plasma and the CSF. The choroid plexus epithelial cells also prevent the spread of infectious agents and other blood-borne entities to thebrain tissue.Chapter topics range from the production of CSF by electrolyte regulation in the choroid plexus, to details on the selectively transporting nature of this barrier.Further, the authors elaborate on the important roles of CSF in sustaining brain health by providing hydration, solutes, and nutrients to the brain tissue. Readers will also learn how CSF circulates signaling molecules within the compartments of the brain and removes waste products from the brain tissue. Elucidating the regulation of these processes in the choroid plexus is not only important for the readers' understanding of normal brain development and function, but is also crucial for resolving a variety of cerebral challenges that lead to brain edema, as well as developing treatments for diseases. The book discusses disease models like hydrocephalus, sleep disorders, and age-related dementia. Its comprehensive coverage makes this volume a valuable resource for researchers in cell and neurophysiology, as well as graduate students of the neurosciences. 296 pp. Englisch.
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EUR 117,69
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details protocols for the use of the biolistic DNA delivery method in different plant species. Chapters guide readers through non-protocol chapters that cover relevant topics of interest, a broad overview of the field, exc…iting modifications of the system, and reliable plant transformation procedures in different plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Biolistic DNA Delivery: Methods and Protocols aims to provide a comprehensive collection of protocols to intended to be a practical guide for the novice as well as the advanced user in the field of plant genetic transformation. 348 pp. Englisch.
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EUR 117,69
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume looks at the methodology and techniques used by experts to study how certain molecules function in specific locations, and their temporal patterns. Chapters in this book cover topics such as in vivo single-molecule trackin…g of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by quantum-dot single particle tracking; SPoD-OnSPAN; and investigating molecular diffusion inside small neuronal compartments with two-photon fluorescence correlation spectroscopy. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and comprehensive, Single Molecule Microscopy is a valuable resource for any researcher interested in learning more about this important field. 348 pp. Englisch.
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EUR 117,69
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents methods used for the analysis of glycoproteins at different levels-intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins… may come across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact proteins to the glycan level, for both N-linked and O-linked glycoproteins; mass spectrometry imaging methodology for glycosylation analysis in tissues; approaches to characterizing glycosylation on cultured cells; and the use of cloud computing to deploy mass spectrometry data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Mass Spectrometry of Glycoproteins: Methods and Protocols is a valuable resource for scientists interested in learning more about this developing field. 416 pp. Englisch.
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EUR 123,04
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This third edition provides revised and expanded protocols of consolidated approaches as well as new trends in the field. Chapters guide readers through new approaches to optimize Quantum Dots' (QD) properties, to evaluate their quant…um yields, important features about preparative processes and characterizations of QDs, methods related to QDs for live cell applications, and the versatility of QDs in the bioanalytical and biosensing field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 320 pp. Englisch.
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EUR 128,39
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume deliversthe most important laboratory techniques that underlie dermatological investigation, includingin situ,in vitro,ex vivo, andin vivomodels of skin, its appendages (hair follicles, sebaceous and sweat glands), and imm…une cells. Also included areunique techniques that unravel epigenetic mechanisms in skin and hair follicle cells, such as gene organization in the nucleus and gene expression regulation by the non-coding transcriptome, which are important recent developments in investigative dermatology. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical,Molecular Dermatology: Methods and Protocolsserves asan essential resource for researchers working in the areas of fundamental as well as applied dermatological science. 288 pp. Englisch.
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EUR 139,09
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of me…thods for over-expression and purification, tools to characterize membrane proteins within different phospholipid bilayers, direction on functional studies, and approaches to determine the structures of membrane proteins. Detailed experimental steps for specific membrane proteins with critical notes allow the protocols to be modified to different systems. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of practical information and implementation advice that leads to excellent, reproducible results.Authoritative and up-to-date, Structure and Function Studies of Membrane Proteins serves as an ideal guide for biologists, biochemists, and biophysicists striving to further understand these essential proteins and their many biological roles. 372 pp. Englisch.
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EUR 139,09
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This third edition provides new and updated chapters detailing a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins with their natural ligands, drugs or other binding par…tners. Chapters detail natural substrates, potential drug leads, quantitative understanding of the mechanism of interaction, and different techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and accessible,Protein Ligand Interactions: Methods and Applications, Third Editionserves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions. 504 pp. Englisch.
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EUR 149,79
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book provides basic information and different protocols associated with the Entomopathogenic nematodes (EPNs) and their symbiotic bacteria. Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis and their associ…ated bacterial symbionts Xenorhabdus and Photorhabdus aid nematode infective juveniles (IJs) in infecting and killing their insect hosts, creating a unique tripartite complex of host-vector-symbiont interactions. Due to this insect killing capability, EPNs are used as biological control agents of economically important insect pests. They are also a model system to study host-parasite interactions.It provides a systematic approach to various nematode procedures including pathogenicity, reproduction, foraging behaviour. It gives a brief outline on historical aspects, nematode-bacterium complex, biology and chemical ecology of EPNs. It concisely describes host insect rearing, nematode samplingand storage, isolation techniques, counting, handling and staining of nematodes, characterization including morphological, molecular and ecological studies, mass production, virulence bioassay, field application and efficacy. The book also includes methods and techniques for their associated symbiotic bacteria.This book serves as a laboratory manual and assists the readers to undertake advanced research in different aspects related to nematodes. It is useful for researchers in the fields of nematology, microbiology, bacteriology and entomology. 192 pp. Englisch.
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EUR 171,19
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book explores the utilization and delivery of stem cells for therapeutic purposes in patients in the clinic and the tightly controlled Good Manufacturing Practices (GMP) that make these powerful techniques possible. Fully updat…ed and expanded from the first edition, this collection features a new set of protocols to arm stem cell biologists with protocols that are currently being used in a number of well-established facilities around the world. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up to date, Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations, Second Edition serves as an ideal guide for researchers aiming to drive continued improvements to GMP and accelerate new stem cell treatments for patients in need. 300 pp. Englisch.
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EUR 171,19
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents methods used for the analysis of glycoproteins at different levels-intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins may co…me across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact proteins to the glycan level, for both N-linked and O-linked glycoproteins; mass spectrometry imaging methodology for glycosylation analysis in tissues; approaches to characterizing glycosylation on cultured cells; and the use of cloud computing to deploy mass spectrometry data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Mass Spectrometry of Glycoproteins: Methods and Protocols is a valuable resource for scientists interested in learning more about this developing field. 416 pp. Englisch.
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EUR 171,19
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume describes a range of methods used in complementlaboratories and how to interpret the data.Chapters detail methods for depletion of IgG and IgM, quantification of complement proteins, C3dg quantification, complement C3 deposition…on endothelial cells, anti-C1q auto-antibodies, and methods for assessment of interactions of proteins with heme. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and clearly written, The Complement System: Innovative Diagnostic and Research Protocols aims to ensure successful results in the further study of this vital field. 280 pp. Englisch.
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EUR 181,89
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides a collection of protocols for the most common experimental methods used for engineeringYarrowia lipolytica. Chapters detail the basic theories underlying the methods described in each chapter. Written in the highly succe…ssful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Yarrowia lipolytica: Methods and Protocols aims to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully. 260 pp. Englisch.
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EUR 181,89
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology. Chapters guide readers through customized dedicated protocols and technologies that will be of valuable help decip…her the numerous functions of small non-coding RNAs. Written in the highly successfulMethods of Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.Instructive and practical,Small Non-Coding RNAs: Methods and Protocols, Second Editionaims to be a valuable help and of great interest to a large number of researchers working in the field of RNA or willing to develop RNA studies. 280 pp. Englisch.
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EUR 181,89
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details protocols on rationale design of therapeutic siRNA molecules and its encapsulation with smart vehicles to overcome the barriers to an effective administrationin vivo. Written in the highly successful Methods in Molecular…Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Design and Delivery of SiRNA Therapeutics aims to ensure successful results in the further study of this vital field. 484 pp. Englisch.
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EUR 171,19
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Buch. Zustand: Neu. This item is printed on demand - Print on Demand Titel. Neuware -The Italian mathematician Mario Pieri (1860-1913) played a major role in the development of algebraic geometry and foundations of mathematics around the turn of the twentieth century. This volume is the second in a series intended to make Pieri'…s research in diverse fields-mathematical logic and philosophy of mathematics, foundations of projective, inversive, and elementary geometry, algebraic and differential geometry, and vector analysis-accessible to today's scholars and to assess its importance (yet little recognized) in historical and modern contexts.The Legacy of Mario Pieri in Foundations and Philosophy of Mathematics examines Pieri's underlying philosophy of mathematics and his research goals, highlighting one of his most influential achievements, his axiomatizations of projective and elementary geometry. Its chapters include three of Pieri's pioneering works, translated by the authors and appearing in English for the first time, as well as an analysis of the role of this research in its larger context. Together, these translations and the accompanying interpretation accurately capture Pieri's expository style, philosophical perspective, and his foundational research, which has influenced generations.With an excellent index, exhaustive references, and engaging discussions that reveal Pieri's work in its historical context, this volume will appeal to scholars and advanced students interested in the history and philosophy of mathematics and logic, as well as readers with a general knowledge of geometry at the intermediate level.A list of errata can be found on the author Smith's personal webpage.Springer Nature c/o IBS, Benzstrasse 21, 48619 Heek 628 pp. Englisch.
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EUR 213,99
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides a comprehensive set of protocols that can be used by any research lab to investigate diverse functional and structural properties of Single Stranded DNA Binding Proteins (SSBs) from eubacterial, archaeal, eukaryotic, mit…ochondrial and viral systems. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Single Stranded DNA Binding Proteinsaims to be auseful practical guide to researchers to help further their study in this field. 388 pp. Englisch.
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EUR 213,99
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods f…or over-expression and purification, tools to characterize membrane proteins within different phospholipid bilayers, direction on functional studies, and approaches to determine the structures of membrane proteins. Detailed experimental steps for specific membrane proteins with critical notes allow the protocols to be modified to different systems. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of practical information and implementation advice that leads to excellent, reproducible results.Authoritative and up-to-date, Structure and Function Studies of Membrane Proteins serves as an ideal guide for biologists, biochemists, and biophysicists striving to further understand these essential proteins and their many biological roles. 372 pp. Englisch.
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EUR 213,99
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The aim of this volume is to provide a comprehensive description of methods and protocols useful for the further study of T-helper cells. Chapters guide readers through T-helper cell recovery, molecular study, signal transduction pathways, T…-cell manipulation and, last but not least, 'omic' approaches. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,T- Helper Cells: Methods and Protocolsaims to be a useful practical guide to researches to help further their study in this field. 400 pp. Englisch.
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EUR 213,99
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This third edition provides new and updated chapters detailing a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins with their natural ligands, drugs or other binding partners.…Chapters detail natural substrates, potential drug leads, quantitative understanding of the mechanism of interaction, and different techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and accessible,Protein Ligand Interactions: Methods and Applications, Third Editionserves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions. 504 pp. Englisch.
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details the most up-to-date technologies used in plant transposable element studies and provides easy-to-follow protocols. Chapters guide readers on available database resources, annotation of different families of transposon, an…d experimental methods to detect their transposition intermediates, neo-transposed DNAs, and transposition events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Plant Transposable Elements: Methods and Protocols aims to provide web-lab and dry-lab methodologies targeted at various levels from beginner to experienced. 288 pp. Englisch.
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EUR 234,33
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents a series of experimental protocols for glycan analysis using lectins and the functional analysis of lectins. A key feature in this book are two comprehensive overview chapters on lectin structures and a list of th…e all the lectins-these chapters discuss the latest findings in the field. The remainder of the book covers topics such as lectins from mammals, non-mammalian animals; plants, algae, fungi, protists, bacteria, and viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Lectin Purification and Analysis: Methods and Protocols is a valuable tool for any scientist interested in learning more about these life systems. 720 pp. Englisch.
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EUR 235,39
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This third edition volume expands on the previous editions with updated chapters on longitudinal studies, randomized trials, evidence-based decisions making, and a new section on changing health-related behaviors. The chapters in this book a…re organized into six parts: Part One focuses on framing clinical research questions and choosing a suitable design; biases that may occur in clinical research; and the ethics associated with doing conducting research on humans. Parts Two through Four discuss designs, measurements, and analysis that pertain to evaluation of risk in longitudinal studies; assessment of therapy in controlled trials; and evaluation of diagnostic tests. Part Five presents methods used in various components of evidence-based decision-making; and Part Six highlights interventions focused on changing health-related behaviors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of various types of bias, step-by-step, readily reproducible protocols for different research designs, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Clinical Epidemiology: Methods and Protocols, Third Edition is a valuable resource for clinicians and researchers who want to expand their works to humans and use their findings in the health system. 664 pp. Englisch.
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EUR 246,09
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book aims to provide an overview of novel bioinformatics resources for exploring diverse aspects of RNA biology. This edition focuses on methods dealing with non-coding RNA (miRNAs, circRNAs or lncRNAs), RNA modifications (m6A…or RNA editing), single cell RNA-seq and statistical models to handle count data from RNA-seq experiments. The book also includes chapters based on the classical RNA bioinformatics methods, such as those for deciphering secondary and tertiary RNA structures; however, they are revised to take into account deep sequencing data. Finally, chapters describing methods to analyze RNA sequencing data from emerging third generation sequencing technologies that could provide interesting insights into the transcriptional process and its regulation are also included.Written for the highly successful Methods in Molecular Biology series, chapters include the kind of expert implementation advice that encourages quality results. Comprehensive and up-to-date, RNA Bioinformatics, Second Edition serves as an ideal guide for researchers digging ever-deeper into the depths of the study of RNAs.The chapter 'RNA-Seq Data Analysis in Galaxy' is open access under a CC BY 4.0 license. 600 pp. Englisch.
