Springer us dez 2021 (43 Ergebnisse)
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book explores techniques used in research efforts to adopt and combine the best capabilities from natural and artificial vector systems to assemble improved delivery technologies aimed for specific applications. The coll…ection discusses vectors other than the traditional viral and non-viral systems and explores ideas for blending the best features of bacteria, nanoparticles, peptides, and hybrid systems for the delivery of biomaterials into cells, as well as a chapter on the application of shotgun proteomics and mass spectrometry as a tool to analyze the proteomic profile changes in cells that result from these interventions. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Bio-Carrier Vectors: Methods and Protocols serves as an ideal guide for researchers working toward harnessing the power of multiple vectors in gene and drug delivery. 256 pp. Englisch.
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details the most up-to-date cryo-EM techniques from leading researchers. Chapters are organized into four parts with emphasis on electron cryotomography, single particle analysis, and the crystal based cryo-EM methods of 2…D electron crystallography, and MicroED for the study of 3D crystals. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, CryoEM: Methods and Protocols aims to serve as an excellent resource on cryo-EM and can serve as the foundation for new researchers to this growing field in structural biology. 364 pp. Englisch.
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the basic issues of 'allostery' and 'network' that are fundamental to studying this field. Chapters in this book look at how the basic 'machine-like' proteins, that are similar to 'human machines,' need to be orga…nized, architecturally, to relate to different organizational layers. Chapters cover topics such as methodological/computational factors focused on links between allostery and network formalism; the presence of oscillating modes transversing the structure and underlying network wiring of the allosteric process; the 'action at distance' by transduction of signals across an organized network structure; and the P53 protein located at the cross-road of cell cycle regulation, genome integrity, and cancer development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and practical, Allostery: Methods and Protocols is a valuable resource for any scientists and researcher interested in learning more about this developing field. 288 pp. Englisch.
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This handbook provides a concise, state-of-the-art overview on the management of inflammatory bowel disease (IBD) in the hospitalized patient. The book reviews epidemiology and risk factors for hospitalization in patients with ulcerat…ive colitis (UC) and Crohn's disease, as well asmedicaland surgical management strategies for patientshospitalizedwith UC orCrohn's disease.The book also highlights complex disease management cases involving co-infection from clostridium difficile and/or CMV. The volume also spotlights nutrition management, quality of care, and management of pediatric and pregnant patients hospitalized with IBD flares. 304 pp. Englisch.
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book explores techniques for understanding and engineering programs that naturally control and drive formation of tissues and organs in order to open powerful opportunities to produce physiologically relevant tissues of…interest, generate models to study human disease, and set the path for the manufacturing of advanced tissue and organs. Beginning with chapters to help understand signaling events and patterns in morphogenetic systems, the volume continues by covering programming signaling events and patterns to drive morphogenesis, techniques for engineering organoids, tissue barriers, and disease models, as well as in vivo therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Programmed Morphogenesis: Methods and Protocols aims not only to communicate knowledge but also to inspire approaches to new challenges and to empower readers with the capability to approach those challenges. 300 pp. Englisch.
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume presents a comprehensive bioinformatic and experimental toolbox for prioritizing, annotating, and functionally analyzing long non-coding RNAs (lncRNAs). Playing a vital role in diverse biological progresses and hu…man disease, lncRNAs have proven to be a challenging subject of study due to our limited understanding of their sequence-function relationships, lack of complete genetic annotation, and the unavailability of systems required to define their functional importance and molecular mechanisms, all of which this book seeks to address. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Functional Analysis of Long Non-Coding RNAs: Methods and Protocols provides a timely and convenient resource to facilitate the identification and characterization of disease-associated human lncRNAs, which aims to shed light on their role in biology and pathophysiology and ultimately lead toward novel therapeutic approaches targeting lncRNAs for the amelioration of human diseases. 368 pp. Englisch.
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides a guide on nanoformulations and other drug delivery approaches for both academic and industry scientists. The chapters in this book cover diverse topics and techniques in nanoparticle drug delivery, gene therapy,…neurosurgical brain implant, exosomes, MRI-guided focused ultrasound (MRgFUS), and advanced preclinical glioblastoma multiforme animal models. Some chapters discuss state-of-the-art and innovative nanomedicines for glioblastoma, surface-modified nanoparticle drug carriers for brain cancer treatment, focused ultrasound (FUS)-mediated blood-brain barrier disruption for enhanced drug delivery to brain tumors; gene therapy delivery approaches to treat brain cancer, and a liposome-template hydrogel nanoparticles (LHNPs) as a powerful CRISPR/Cas9 delivery vehicle. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and thorough, Nanotherapy for Brain Tumor Drug Delivery is a valuable resource for the scientific community working on this important therapeutic field, and will help fast-track the clinical translation of revolutionary nanotechnologies for treating brain tumors. 312 pp. Englisch.
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EUR 128,39
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume encompasses short targeted reviews and methods featuring the construction and use of glycan microarray platforms. Chapters guider readers through glycan microarrays comprised of synthetic glycans, natural biomolecules, bac…teria, neoglycoproteins, various applications and progress of these platforms.Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge,Glycan Microarrays: Methods and Protocols aims to highlight the interdisciplinary nature of the development and use of the glycan microarray platform. 268 pp. Englisch.
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume aims at providing a selection of the most effective and widely employed protocols used in the Ewing sarcoma field. Chapters are divided into five sections detailing themolecular biology techniques used to analyze Ewing sar…coma including tissue preservation and methods used in morpho-molecular diagnostics and routine immune-histological analysis. Additional wet-lab protocols comprise in vitrotechniquesincluding re-expression and functional analyses of target genes together within vivotools and models such as patient-derived xenografts (PDX) and orthotopic xenografts for metastasis assessment in Ewing sarcoma.A full section on bioinformatic approaches to Ewing sarcoma covering ChIPseq, epigenetic and systems biology analyses finalizes this volume.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and clearly written,Ewing sarcoma: Methods and Protocolsaims to provide methods to further the study ofEwing sarcoma and bring researchers one step forward in the quest to fight this devastating disease. 352 pp. Englisch.
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fully updated edition explores conceptual as well as technical guidelines for plant taxonomists and geneticists, such as the increasing use of next-generation sequencing (NGS) technologies for numerous applications in plant taxon…omy. The volume provides molecular approaches to be used within an 'integrative taxonomy' framework, combining a range of nucleic acid and cytogenetic data together with other crucial information (taxonomy, morphology, anatomy, ecology, reproductive biology, biogeography, paleobotany, etc.), which will help not only to best circumvent species delimitation but also to resolve the evolutionary processes in play. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Molecular Plant Taxonomy: Methods and Protocols, Second Edition is an ideal guide for researchers seeking a better understanding of evolutionary processes, at species and population level, through molecular techniques. 416 pp. Englisch.
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EUR 128,39
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book collects a variety of methods from investigators at the forefront of chondrocyte biology and pathology research that they have tailored for their research projects in order to tackle the unique properties and challe…nges of the chondrocyte and those of its tissue matrix. Divided into three parts, the volume explores experimental models to study chondrocytes, in vivo assays, as well as methods to characterize the chondrocyte phenotype with high-throughput or discrete assays on cells maintained in vivo, just isolated from their tissue (ex vivo), or cultured in vitro. Written in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Chondrocytes: Methods and Protocols is an ideal guide for experts as well as newcomers in the cartilage field with the aim of sparking new research ideas in order to solve many of the pending mysteries regarding cartilage biology, disease mechanisms, and potential treatments. 244 pp. Englisch.
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping a…pproaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Crop Breeding:Genetic Improvement Methodsaims to ensure successful results in the further study of this vital field. 292 pp. Englisch.
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EUR 139,09
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book explores examples of current in vitro and in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane… models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer in silico molecular dynamics modeling, novel techniques to explore lipid bilayer characteristics using neutron scattering, IR spectroscopy, and atomic force microscopy (AFM), as well as unique fluorescence techniques. Written in the highly successful Methods in Molecular Biology series style, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Membrane Lipids: Methods and Protocols serves as an ideal guide for researchers seeking to further investigate the often complicated world of lipid membrane biophysics. 312 pp. Englisch.
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides detailed protocols for the performance, analysis, and troubleshooting ofin vitroandin vivoexperiments related to programmed cell death. Chapters compileconventional techniques such aswestern blot and qPCR and stat…e-of-the art transmission electron microscopy and real-time multiplexed imaging assays. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and accessible,Detection of Cell Death Mechanisms: Methods and Protocolsaims to ensure seamless execution of protocols on specific cell death type. 276 pp. Englisch.
Sprache: Englisch
Verlag: Springer US Dez 2021, 2021
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This manual details the techniques involved in the study of plant microbe interactions (PMI). Covering a wide range of basic and advanced techniques associated with research on biological nitrogen fixation, microbe-mediated plant nutr…ient use efficiency, the biological control of plant diseases and pests such as nematodes, it will appeal to postgraduate students, research scholars and postdoctoral fellows, as well as teachers from various fields, including pathology, entomology and agronomy.It consists of five broad sections featuring different units. Information panels at the beginning of each unit present essential knowledge as well as advances in a particular topic. The manual can also serve as a textbook for undergraduate courses like Techniques for Plant-Microbe Interactions; Biological Control of Plant Diseases; and Nutrient Use Efficiency. Providing basic insights and working protocols from all related disciplines, this unique laboratory manual is a valuable resource for researchers interested in investigating PMI. 320 pp. Englisch.
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of cran…iofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (c. elegans, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant andin vivocell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression usingin-situhybridisation andsingle-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such asCRISPR/Cas9-mediated deletion.Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge,Craniofacial Development: Methods and Protocolsaims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques. 328 pp. Englisch.
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition volume is a companion volume to the previous edition and looks at new findings on novel bioactive chemicals and cognate targets, as well as the use of synthetic small molecules and a variety of tools to understand…these processes. Chapters in this book cover topics such as screening plants for novel bioactive chemicals including lipid signaling and photo receptors; small molecule screens to include peptide ligands to generate new variations; using new chemicals to affect and dissect hormone signaling; and the application of easier microscale methods to simplify target identification and validation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Plant Chemical Genomics: Methods and Protocols, Second Edition is a valuable resource that provides a foundation of techniques for novice and expert researchers in the plant chemical biology community. 208 pp. Englisch.
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents techniques used by researchers from all branches of biology to study daily changes at a molecular level in many physiological systems. The chapters are organized into three parts and cover topics such as measuring… and modeling physiological and behavioral rhythms; genome-wide analyses in circadian biology, and imaging and manipulating brain clocks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Circadian Clocks: Methods and Protocols is a valuable tool for any researcher interested in learning more about this developing field. 364 pp. Englisch.
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores strategies and detailed protocols for the preparation of macromolecular complexes and their characterization in view of structural analysis. The chapters in this book are separated into three parts: Part One focus…es on sample preparation, and covers strategies for recombinant expression of multiprotein complexes in prokaryotic and eukaryotic hosts, for genome engineering using the CRISPR/Cas9 system and for production of specific binders such as reformatted antibodies and artificial binding proteins. Part Two looks at the biophysical methods that can provide useful indicators for sample optimization, and often complement structural information obtained with core technologies for structure determination-x-ray crystallography and cryo-electron microscopy-by quantitative solution data. Part Three discusses the characterization of multiprotein complexes in a cellular environment using the latest technologies and in vivo approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Multiprotein Complexes: Methods and Protocols is a valuable resource for structural and molecular biologists who need to prepare multi-components for their applications, and for other scientists working on macromolecular assemblies from other angles that need to know the latest approaches that the field has to offer. 352 pp. Englisch.
Sprache: Englisch
Verlag: Springer US Dez 2021, 2021
Serie: Buch 53 von 53 - Progress in Nonlinear Differential Equations and Their Applications
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EUR 160,49
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The theory of real-valued Sobolev functions is a classical part of analysis and has a wide range of applications in pure and applied mathematics. By contrast, the study of manifold-valued Sobolev maps is relatively new. The incentive to expl…ore these spaces arose in the last forty years from geometry and physics. This monograph is the first to provide a unified, comprehensive treatment of Sobolev maps to the circle, presenting numerous results obtained by the authors and others. Many surprising connections to other areas of mathematics are explored, including the Monge-Kantorovich theory in optimal transport, items in geometric measure theory, Fourier series, and non-local functionals occurring, for example, as denoising filters in image processing. Numerous digressions provide a glimpse of the theory of sphere-valued Sobolev maps.Each chapter focuses on a single topic and starts with a detailed overview, followed by the most significant results, and rather complete proofs. The 'Complements and Open Problems' sections provide short introductions to various subsequent developments or related topics, and suggest newdirections of research. Historical perspectives and a comprehensive list of references close out each chapter. Topics covered include lifting, point and line singularities, minimal connections and minimal surfaces, uniqueness spaces, factorization, density, Dirichlet problems, trace theory, and gap phenomena.Sobolev Maps to the Circle will appeal to mathematicians working in various areas, such as nonlinear analysis, PDEs, geometric analysis, minimal surfaces, optimal transport, and topology. It will also be of interest to physicists working on liquid crystals and the Ginzburg-Landau theory of superconductors. 564 pp. Englisch.
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EUR 181,89
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume encompasses short targeted reviews and methods featuring the construction and use of glycan microarray platforms. Chapters guider readers through glycan microarrays comprised of synthetic glycans, natural biomolecules, bacteria,…neoglycoproteins, various applications and progress of these platforms.Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge,Glycan Microarrays: Methods and Protocols aims to highlight the interdisciplinary nature of the development and use of the glycan microarray platform. 268 pp. Englisch.
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant rout…es to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Human Naïve Pluripotent Stem Cells: Methods and Protocols is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field. 300 pp. Englisch.
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EUR 192,59
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume collects a selection of key techniques for studying cell division, representing multiple model systems and varied scales of approach. Over the past 20 years, a series of revolutions in experimental molecular biology, inc…luding chimeric fluorescent protein expression, multiple advanced modes of quantitative microscopy, and array of small molecule inhibitors, proteomic profiling, and gene silencing/manipulation/analysis, has advanced the mitosis field to a point where single cell biology not only allows for imaging/localization studies, but also for quantitative analysis and sequencing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Mitosis: Methods and Protocols provides a repository of techniques and approaches for those working in the field as well as a working resource for those venturing into the study of mitosis for the first time. 268 pp. Englisch.
Sprache: Englisch
Verlag: Birkhäuser, Springer US Dez 2021, 2021
Serie: Buch 53 von 53 - Progress in Nonlinear Differential Equations and Their Applications
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Buch. Zustand: Neu. This item is printed on demand - Print on Demand Titel. Neuware -The theory of real-valued Sobolev functions is a classical part of analysis and has a wide range of applications in pure and applied mathematics. By contrast, the study of manifold-valued Sobolev maps is relatively new. The incentive to explore…these spaces arose in the last forty years from geometry and physics. This monograph is the first to provide a unified, comprehensive treatment of Sobolev maps to the circle, presenting numerous results obtained by the authors and others. Many surprising connections to other areas of mathematics are explored, including the Monge-Kantorovich theory in optimal transport, items in geometric measure theory, Fourier series, and non-local functionals occurring, for example, as denoising filters in image processing. Numerous digressions provide a glimpse of the theory of sphere-valued Sobolev maps.Springer Nature c/o IBS, Benzstrasse 21, 48619 Heek 564 pp. Englisch.
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Taschenbuch. Zustand: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This detailed book highlights several emerging areas in the implementation of green chemistry in medicinal chemistry drug discovery with a specific focus on their application to the expeditious discovery of new biologically active entitie…s. Divided into three sections, the collection explores greener approaches to chemical transformations that are both prevalent and have been highlighted as challenging within the pharmaceutical industry, overall synthetic strategy, as well as the implementation and impact of a range of enabling technologies within medicinal chemistry. As a volume of the Methods in Pharmacology and Toxicology series, chapters provide the kind of key insight that can guide researchers toward greater success in the lab.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 636 pp. Englisch.
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest techniques used to better understand the brain reward system with respect to neurotransmitters, brain structures, and connectivity. This book aims to show readers tested laboratory protocols to study ne…ural circuitry and biological processes implicated in reward, and in neuropsychiatric disorders such as substance use disorders. The chapters are organized into four parts. Part One addresses classical techniques to study the brain reward system, including the curve shift paradigm in intracranial self-stimulation, stereotaxic surgery in rodents, and the use of brain lesions. Part Two focuses on neurochemical, behavioral, and chemogenetic techniques such as immunofluorescence for assessing adult hippocampal neurogenesis, and fast-scan voltammetry. Part Three highlights methods used to assess the rewarding potential of drugs including intracranial self-stimulation combined with drug injection, and the use of viral vectors. The Fourth Part introduces imaging and electrophysiology techniques such as positron emission tomography, in vivo electrophysiology, and fiber photometry. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and thorough, The Brain Reward System is a valuable resource for researchers interested in learning more about the current methods used to study the delineation of the brain reward system. 380 pp. Englisch.
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents updated methods and new developments in the field of mass spectrometry imaging. Chapters guide readers through four parts covering imaging, software, data analysis, new instrumentation, and new methodological approaches.… Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Mass Spectrometry Imaging of Small Moleculesaims to be auseful practical guide to researchers to help further their study in this field. 288 pp. Englisch.
Sprache: Englisch
Verlag: Springer US Dez 2021, 2021
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores immune cell receptors that are used in the detection of microbes, either by binding directly to non-self molecules or through indirectly sensing microbe-associated cellular disturbances. The chapters in this book cover m…ethods for studying receptor-ligand interactions at both molecular and cellular levels; methods to create and characterize novel antibody reagents; and methods to characterize the molecular processes that lead to adaptive receptor maturation. This book also contains chapters that look at high-throughput strategies that describe the diversity of immune receptors and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Immune Receptors: Methods and Protocols, Second Edition is a valuable resource for scientists and researchers interested in learning more about this developing field. 280 pp. Englisch.
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume explores techniques in clinical proteomics, an emerging discipline aimed at deciphering the molecular mechanisms underlying the progression of diseases and at identifying new biomarkers and therapeutic targets to expand…the physicians' toolbox for precision medicine-based patient care. From sample processing to multi-omics approaches, the book provides straight-forward protocols on a wide array of vital areas of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Clinical Proteomics: Methods and Protocols serves as an ideal guide for researchers working to expand upon the knowledge base needed to push forward toward a more personalized version of medicine. 280 pp. Englisch.
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacia…l development. Chapters guide readers through the use of disparate models to study formation of the head and face (c. elegans, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant andin vivocell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression usingin-situhybridisation andsingle-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such asCRISPR/Cas9-mediated deletion.Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge,Craniofacial Development: Methods and Protocolsaims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques. 328 pp. Englisch.
