Springer us feb 2021 (16 Ergebnisse)
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EUR 117,69
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides current management and research protocols on oesophageal squamous cell carcinoma. Chapters guide readers through methods on clinical and pathological diagnostics, translational research for oesophageal squamous ce…ll carcinoma, in vitro assays, liquid biopsy for cancer DNA and circulating tumour cells, genomic analysis, mi-RNAs, and proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Esophageal Squamous Cell Carcinoma: Methods and Protocols to ensure successful results in the further study of this vital field. 400 pp. Englisch.
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EUR 117,69
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This third volume exemplifies and illustrates exciting advances in peptide nucleic acids (PNA) chemistry, and serves as a vital complement to the first and second edition of the book. Chapters focus on in vivo properties and behavior…and applications of PNA while providing contributions on both chemistry and nucleic acid recognition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Peptide Nucleic Acid: Methods and Protocols, Third Edition aims to ensure successful results in the further study of this vital field. 320 pp. Englisch.
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EUR 117,69
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume looks at the different spectroscopic and biophysical methods used by researchers to study the structure and folding of RNA, and to follow their interactions with proteins. The chapters in this book cover topics such as sin…gle-molecule spectroscopy of multiple RNA species; surface plasmon resonance, MS or microcalorimetry for investigating molecular interactions with RNA; FTIR, SAXS, SANS and SRCD spectroscopies to analyze RNA structure; use of fluorescent nucleotides to map RNA-binding sites on proteins surfaces or CryoEM; and much more. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, RNA Spectroscopy: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field. 368 pp. Englisch.
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EUR 128,39
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 268 pp. Englisch.
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EUR 139,09
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume looks at applications of quantum mechanical (QM) methods in drug discovery. The chapters in this book describe how QM approaches can be applied to address key drug discovery issues, such as characterizing protein-water-lig…and and protein-protein interactions, providing estimates of binding affinities, determining ligand energies and bioactive conformations, refinement of molecular geometries, scoring docked protein-ligand poses, describing molecular similarity, structure-activity-relationship (SAR) analysis, and ADMET prediction.Writtenin the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary software and tools, step-by-step, readily reproducible modeling protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge andunique,Quantum Mechanics in Drug Discovery is a valuable resource for structural and molecular biologists, computational and medicinal chemists, pharmacologists, and drug designers. 372 pp. Englisch.
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EUR 160,49
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume highlights various techniques used to study multiple aspects of mGlu receptor function. Chapters in this book cover topics such as modern approaches for the development and screening of allosteric modulators; fluctuations in intr…acellular calcium induced by mGlu receptor activation; dopamine-glutamate interactions in the central nervous system; methods for assessing mGlu receptor localization; and common techniques for evaluating mGlu modulation in behavior in rodents, including stress and alcohol drinking. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and practical, Metabotropic Glutamate Receptor Technologies provides researchers with a starting point to address important questions regarding mGlu and its importance. Researchers working in the fields of neuroscience and physiology of multiple peripheral systems will find this volume most useful. 328 pp. Englisch.
Sprache: Englisch
Verlag: Springer US Feb 2021 2021
Serie: Springer Protocols Handbooks, Buch 44 von 84. Buch 44 von 84 - Springer Protocols Handbooks
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EUR 165,84
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The book presents hands-on protocols for conventional and advanced forensic DNA fingerprinting experiments. It includes manual, semi-automatic, and advanced automatic techniques for DNA extraction from different biological samples.It…also discusses various qualitative and quantitative approaches for the assessment of extracted forensic DNA. It contains protocols for the amplification of short tandem repeat markers (STRs) for the amplification-based target enrichment of the forensic samples.Further, it examines genotyping of the STR loci through capillary electrophoresis and includes real-world case studies where forensic DNA analysis has been used in the criminal and civil disputes. The book concludes by presenting technological developments in the field of DNA forensic analysis. Suitable for beginners, it is a key reference resource on a wide variety of DNA profiling techniques and applications. 364 pp. Englisch.
Sprache: Englisch
Verlag: Springer US Feb 2021 2021
Serie: Springer Protocols Handbooks, Buch 52 von 84. Buch 52 von 84 - Springer Protocols Handbooks
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EUR 171,19
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book explores protocols for a wide array of preclinical pharmacology and toxicology evaluations to be applied to chemical drugs and their development through in vitro, involving tissues and cell lines, and in vivo models, using… animals as experimental systems, utilized to conduct pharmacological research. Written for the Springer Protocols Handbooks series, the methodologies included in this collection have been standardized by the authors through extensive use in the lab so that they are ready to be applied in the labs of readers around the world.Authoritative and practical, Bioassays in Experimental and Preclinical Pharmacology aims to assist undergraduate and postgraduate students, research scholars, scientists, and other academicians performing research in the vital field of drug discovery. 284 pp. Englisch.
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EUR 176,54
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applicatio…ns. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, RNA Interference and CRISPR Technologies: Technical Advances and New Therapeutic Opportunities is a valuable resource for any scientist, teachers, graduate student, postdoc, and clinician interested in this field. This book also benefits anyone in research and development in biotech and pharmaceutical companies who want to understand more about these technologies, and their applications in biology and medicine. 504 pp. Englisch.
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EUR 181,89
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the techniques used to study human and animal pericytes. Chapters in this volume cover a range of topics such as pericyte dissociation from tissues, and analysis by immunohistochemistry and flow cytometry; avian chimaera…s to investigate the embryonic development of cell lineage; pericyte purification by fluorescence activated cell sorting and ensuing culture; tissue turnover and regeneration; and the dynamic study of pericyte involvement in cancer dissemination, in situ, and in culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Pericytes: Methods and Protocols is a valuable resource that helps researchers define the biologic identity, functions, and potentials of a cell lineage of increasing interest and therapeutic significance. 204 pp. Englisch.
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EUR 192,59
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides recent advances in the field of biophysics of membrane proteins. Chapters are divided into several parts: detailing biochemistry and functional analysis, experimental and theoretical structural determinations, membrane p…rotein dynamics, and conformation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Biophysics of Membrane Proteins: Methods and Protocols aims to provide comprehensive protocols with notes to help further the understanding of key membrane protein structure and function forstudents, academics, and industrial researchers. 348 pp. Englisch.
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EUR 192,59
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book collects original protocols aimed at encouraging and stimulating the scientific community to design and produce models for the laboratory that mimic cell guidance conditions as they occur in vivo. The protocols collected d…escribe powerful strategies to exploit chemical cues involved in cell differentiation processes. Special emphasis is given to the use of methods for purification and characterization of exosomes and other secreted vesicles, as well as micro and non-coding RNAs, that have been demonstrated to control the tuning of the in vivo micro and macro environment in order to ensure the optimal soluble environment in vitro. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Next Generation Culture Platforms for Reliable In Vitro Models: Methods and Protocols serves as an ideal guide for researchers working toward developing these vital testing models for preclinical studies. 320 pp. Englisch.
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EUR 192,59
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fully updated edition provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulatio…n of genes of interest, tracing cell lineages, and modeling human diseases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Mouse Genetics: Methods and Protocols, Second Edition delivers fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students, and postdoctoral fellows working in the various disciplines of genetics, developmental biology, mouse genetics, and modeling human diseases. 232 pp. Englisch.
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EUR 235,39
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition provides new and updated chapters from expert researchers in the field detailing methods used to studythe multi-facet deep sequencing data field. Chapters guide readers through techniques for processing RNA-seq data, micr…obiome analysis, deep learning methodologies,and various approaches for the identification of sequence variants.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Deep Sequencing Data Analysis: Methods and Protocols, Second Editionaims to ensure successful results in the further study of this vital field. 384 pp. Englisch.
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details a wide range of methods, ranging from beginner through advanced, used tofurther study zebrafish and fish germline. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and t…he molecular analysis of their, protein, and RNA. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Germline Development in the Zebrafish: Methods and Protocols aims to ensure successful results in the further study of this vital field. 400 pp. Englisch.
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EUR 246,09
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Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This new edition explores and provides an update on the biology and pathogenesis of human cytomegalovirus infection. Modern techniques that are currently being utilized to investigate the molecular aspects of viral infection, as well as how…these new research studies are leading to new approaches to mitigate disease, are also provided. Given the key role the virus plays in significant acute and chronic human disease in all stages of life, from newborns to seniors, the need for clear methodologies to further explore the biology of HCMV infection and mitigation strategies is readily apparent. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Human Cytomegaloviruses: Methods and Protocols, Second Edition serves as an ideal scientific reference for basic and clinical scientists and medical personnel on the modern understanding of the pathobiology of the virus, and the approaches, techniques, and models to study human cytomegalovirus infection and disease. 480 pp. Englisch.
