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Taschenbuch. Zustand: Neu. Neuware -Cutting-edge and thorough, Spectral and Imaging Flow Cytometry: Methods and Protocols, Second Edition is a valuable resources for beginners and professionals interested in learning more about this developing field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 276 pp. Englisch.

Buch. Zustand: Neu. Neuware -This second edition provides new and updated methods for establishing reliable and reproducible experimental models of cardiovascular diseases. Chapters detail practical protocols from expert laboratories focusing on cardiovascular research, that would be critical in exploring novel discoveries in cardiac biology, and the development of effective therapeutic approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Experimental Models of Cardiovascular Diseases: Methods and Protocols, Second Edition aims to provide detailed and practical protocols that will be valuable tools for researchers in cardiology to conduct their research.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 272 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -This new collection features the most up-to-date essential protocols that are currently being used to study the immune synapse. Beginning with methods for making biophysical measurements, the volume continues by covering the cell biology of synapses, methods for advanced substrate engineering, mechanobiology topics, new technologies to describe and manipulate synaptic components, as well as methods related to sites of action and immunotherapy. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 532 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -Tracing the life of a giant in inorganic chemistry and key trends in his science, Boranes and Beyond follows Hawthorne from his mid-American origins to the halls of Harvard and UCLA and back again. It naturally details the accomplishments in his lab. This book is a fascinating mixture of science and autobiography. Prof. Hawthorne won the Priestley Medal, the highest award of the American Chemical Society, for his pioneering work in elucidating the chemistry of boron. He has chronicled in this book the developments in his lab which ultimately led to this achievement.Not content to rest on his laurels, after retiring from UCLA Prof. Hawthorne explored the use of boron in biomedicine and directed the International Institute of Nano & Molecular Medicine at the University of Missouri-Columbia.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 288 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume explores the latest developments in the study of connexin hemichannels (HCs). The chapters in this book cover topics such as purification, reconstitution, and functional analysis of connexin HCs; spatial and temporal localization of connexins in cells using confocal microscopy; evaluation of connexin HC activity in vivo; generation of connexin-expressing stable cell pools; and automated assessment of cutaneous pathology in a mouse model of HC dysfunction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Connexin Hemichannels: Methods and Protocols is a valuable resource that discusses the importance of HCs as drug targets, and how studying them is essential to identifying newcompounds capable of modulating HC function selectively and specifically.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 216 pp. Englisch.

Buch. Zustand: Neu. Neuware -This detailed collection explores thermomorphogenesis, a discipline that unravels the intricacies of warm temperature responses in plants. How plants perceive, interpret, and respond to elevated temperatures has far-reaching implications for agriculture, ecology, and our fundamental understanding of plant development, and this book presents techniques to help researchers delve into this area of study. Beginning with a section on thermomorphogenesis responses, the volume continues with numerous chapters on temperature sensing and temperature signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 280 pp. Englisch.

Buch. Zustand: Neu. Neuware -This detailed volume explores the dynamic landscape of patient-derived xenograft (PDX) generation and applications. After a few chapters examining the evolution of PDXs, tracing their roots and contextualizing their significance in the broader narrative of cancer research, the book continues with methodologies for generating PDXs from diverse clinical tissue samples as well as for utilizing PDXs in various specific cancer types. From the development of humanized PDX models to the exploration of protein expression through advanced techniques including multiplex immunohistochemistry, Western blotting, and immunoprecipitation, the chapters offer a comprehensive toolkit for researchers and clinicians alike. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 256 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume discusses different aspects of immune homeostasis regulation and its dysregulation during various diseases, including infections, cancers, neurodegenerative diseases, autoinflammation, autoimmune diseases, and immunodeficiency, along with immunological, genetic, cell biology, and molecular biology techniques designed to understand immune homeostasis at different stages. The chapters in this book cover topics such as T cell subsets; monocyte-myofibroblast immunomodulation in a 3D co-culture system; human peripheral blood mononuclear cells in patients with neurodegenerative diseases; and CRISPR-Cas9 induced gene-editing in primary human monocytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 248 pp. Englisch.

Buch. Zustand: Neu. Neuware -This detailed volume presents methodologies for studying reactive oxygen species (ROS) metabolism in plant cells, including techniques for detecting different types of ROS such as superoxide radicals, hydrogen peroxide or singlet oxygen, as well as ROS generating systems such as xanthine oxidoreductase. The book provides alternatives to determine malondialdehyde, electrochemical detection approaches for determining total antioxidant capacity (TAC) as well as enzymatic antioxidants, as well as methods for studying non-enzymatic antioxidants and some components of the secondary metabolism. Written for the highly successful Methods in Molecular Biology series, the chapters in this book provide the kind of detailed implementation advice that leads to ideal results in the laboratory.Authoritative and practical, ROS Signaling in Plants: Methods and Protocols serves as an ideal guide for scientists interested in the metabolism of ROS in plant cells. 266 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume details protocols ranging from high yield production metabolically labeled KRAS for NMR studies to approaches that quantify engagement of novel molecules that bind KRAS in live cells. Chapters focus on protein production and characterization, biochemical assays, cell-based assays, KRAS-membrane interactions, targeting KRAS, and cell models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, KRAS: Methods and Protocols aims to provide methods that will be instrumental in the development of future clinically approved KRAS therapeutics.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 380 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume explores the latest developments in the study of the cerebral cortex. The chapters in this book cover a wide array or established methods such as in situ hybridization of brain slices; two-photon FRET/FLIM imaging of cerebral neurons; BioID analysis of actin-binding proteins; generation of iPS cells using Sendai Virus vectors; in vivo whole-cell recording from the mouse brain; and locomotor assays in Drosphila larvae and adult flies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and thorough, Cerebral Cortex Development: Methods and Protocols is a valuable resource that will help both expert and novice researchers further enhance their understanding of this important field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 368 pp. Englisch.

Buch. Zustand: Neu. Neuware -This fully updated edition explores fundamental protocols for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Featuring protocols to examine light response curves and gas exchange measurements for a variety of samples, the book includes new protocols on photosynthesis in the adaxial and abaxial sides of a leaf, non-foliar organs, and aquatic systems. Protocols and best practice for eddy covariance, thermal, spectral, and hyperspectral imaging and data analysis, as well as stable isotope labeling and quantification of photosynthetic metabolites, are introduced alongside fresh insights on many more topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 484 pp. Englisch.

Buch. Zustand: Neu. Neuware -This detailed book gathers hands-on techniques to characterize, identify, localize, profile, and determine genome functions in plants. Volume 1 opens with a section exploring protocols to advance our understanding of plant genetics and ecology via phenomics, and it continues with chapters on plant genetics, transcriptomics, and proteomics to unravel plant protein structures and functions, bridging proteomics and phosphoproteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 372 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume covers the latest advancements and methods used to study DNA barcoding. The chapters in this book are organized into six parts: theory, DNA manipulation, specialized barcoding methods, analysis of DNA barcodes, completing a successful DNA barcode project, and barcoding as citizen science. Part One looks at several theoretical issues in DNA barcoding concerned with DNA barcodes that can and should be used. Part Two discusses protocols for processing samples into DNA barcodes, including DNA isolation, sequencing and managing samples, and the resulting DNA barcodes. Part Three explores the next generation of DNA barcoding such as FRET-based detection, dual nanopore detection, skimming, and closed-tube methods. Part Four addresses programs and websites that handle DNA barcoding data, and includes character-based approaches, barcode gap methods, integrated approaches, and Barcode of Life Database. Part Five offers protocols for producing a successfulDNA barcoding project including plant and animal examples and protocols for generating a DNA Barcode Reference Library. Lastly, Part Six talks about several novel protocols for setting up an educational program using DNA barcodes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, DNA Barcoding: Methods and Protocols encompasses a wide range of techniques and will be a useful reference tool for both scientist and student.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 596 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume explores emerging approaches and methods inspired by recent research using ctenophores or placozoans, facilitating the study of these enigmatic species and ancient animal lineages. Practical protocols are integrated with introductory chapters about general biology, summarizing the diversity, evolution, genomics, development, and neurobiology of these organisms. Special chapters outline strategies and applications for culturing, microscopy, physiology, electrical synapses, and bioluminescence, molecular and single-cell biology, transcription factors, as well as gene and protein expression analyses. The book also contains bioinformatics and computational chapters covering peptidomics and epigenomics. Written for the highly successful Methods in Molecular Biology series, chapters include detailed implementation advice that leads to dependable results in the laboratory.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 600 pp. Englisch.

Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents a broad collection of state-of-the-art methods to study the biology of thermogenic fat using in vitro cell culture and animal models. Chapters guide the readers through protocols on differentiation of human pluripotent stem cells and murine adipocyte precursors; methods for measuring mitochondrial respiration, heat generation, brown fat activation, and effects on energy metabolism in mice; and techniques for AAV-mediated gene delivery, transplantation of adipose tissue, isolation of adipose tissue immune cells and extracellular vesicles, and mass spectrometry-based profiling of brown fat lipids. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge,Thermogenic Fat: Methods and Protocols aims to be comprehensive guide for researchers in the field. 268 pp. Englisch.

Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details critical and valuable experimental protocols to facilitate researchers in this fascinating field. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Maternal Placental Interface: Methods and Protocols aims to ensure successful results in the further study of this vital field. 208 pp. Englisch.

Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This third edition volume expands on the previous editions with new and updated discussions on the latest developments in endotoxin contamination, complex physicochemical properties, in vitro immunotoxicity traits, and in vitro drug release properties. Eight chapters in this book are dedicated to physicochemical characterization techniques and cover newer methods such as asymmetric-flow field-flow fractionation, single particle inductively coupled plasma mass spectrometry, and resistive pulse sensing. The next eighteen chapters explore the immunotoxicity of nanomaterials, including microbial contaminants such as endotoxin and beta-glucans, anti-PEG antibodies, autoimmunity, and immunosuppressive properties. The last two chapters talk about new pharmacology protocols, including a new technique to assess drug release and a tissue distribution assay using PEG immunohistochemistry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step instructions to reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Characterization of Nanoparticles Intended for Drug Delivery, Third Edition is a valuable resource that will help both expert and novice researchers further enhance their understanding of this important and developing field. 336 pp. Englisch.

Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest advancements and techniques used by researchers to work with plant parasitic nematodes (PPNs) and to study their interactions with crop plants. The chapters in this book cover topics such as ways to obtain samples from soil and different plant tissues; rearing nematodes in greenhouse setting; methods used to axenically rear nematodes in vitro; Denaturing Gradient Gel Electrophoresis (DGGE) separation; methods for ROS detection and hydrogen peroxide quantification; methods to elicit plant resistance against nematodes; methods for the detection of Pathogenesis Related (PR) genes that are the executioners of immunity in plant-RKN interactions; and laboratory procedures that test nematodes on their isolation, count, culture, identification, pathogenicity, virulence, and environmental tolerance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Plant-Nematode Interactions is a valuable tool for any researcher interested in learning more about this interesting and developing field. 400 pp. Englisch.

Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 332 pp. Englisch.

Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition provides new and updated methods detailing techniques and state-of-the-art approaches on the structure and function of plant endoplasmic reticulum (ER). Chapters guide readers through modern microscopy techniques, software protocols, purification, and analysis of ER membrane structure. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,The Plant Endoplasmic Reticulum: Methods and Protocols, Second Editionaimstoensure successful results in the further study of this vital field. 452 pp. Englisch.

Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Since the publication of the previous edition, the use of 3D cell and organoid cultures has become more widespread across laboratories. This second edition volume expands on the previous edition with discussions about the latest organoid models developed for many more organs; new hydrogels and devices for 3D culture; and the organoid systems that have been improved by incorporating more components of tissue microenvironments in the in vitro culture. The chapters in this book are organized into five parts and cover topics such as biofabrication, organoids, microfluidic systems, bioprinting, and image analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and cutting-edge, 3D Cell Culture: Methods and Protocols, Second Edition is a valuable resource that will stimulate new ideas, foster interdisciplinary collaborations, and contribute to the improvement of human health and well-being. 348 pp. Englisch.

Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest technologies used to study the co-existence of neuronal and neuron-glia synapses in the brain. The chapters in this volume are organized into three parts. Part One covers recent advancements in the technical approaches for studying glutamatergic signalling between neurons. Part Two highlights approaches to study the functional role of astrocytes at neuronal synapses. Part Three focuses on fast signalling at neuron-glia synapses, and highlights relevant methods for investigating these unusual synapses, including slice electrophysiology and in vivo gene delivery techniques. In the Neuromethods series style, all chapters contain key advices from experts in the field that are valuable for performing successful experiments on the topic in your laboratory.Cutting-edge and thorough, New Technologies for Glutamate Interactions: Neurons and Glia is a valuable resource for both neuronal and glial physiologists who are interested in learning about and developing new methods to further understand neuronal and neuron-glia synaptic transmission. 388 pp. Englisch.

Buch. Zustand: Neu. This item is printed on demand - Print on Demand Titel. Neuware Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 432 pp. Englisch.

Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest developments in the study and application of phage biology. The chapters in this book are divided into five parts and cover topics such as phage display, selection, and evolution; genetic and chemical modification of phages; analyzing structures by electron microscopy; characterizing phage transcripts and proteins; and the biology of whole phages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Phage Engineering and Analysis: Methods and Protocols is a valuable resource that will help both expert and novice researchers - from backgrounds ranging from microbiologists to biophysicists to chemical engineers - further enhance their understandingof this important and evolving field. 312 pp. Englisch.

Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest techniques and protocols used by researchers to address unique biological questions, model organisms not typically studied by Fluorescent In Situ Hybridization (FISH), protocols combining FISH with immunofluorescence (FISH-IF), and high-throughput experiments. The chapters in this book are divided into two parts: RNA FISH protocols and DNA FISH protocols. Part One covers methods for designing OligoPaint probes and studying distinct aspects of RNA biology such as transcription and splicing dynamics, and mRNA and small RNA expression and localization. Part Two discusses DNA repair dynamics, gene compaction, and chromatin conformation and gene rearrangements in plants, insects, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and thorough, Fluorescence In Situ Hybridization (FISH): Methods and Protocols is a valuable resource that will benefit the broader scientific community in their studies and understanding of this important field. 316 pp. Englisch.

Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest advancements and techniques to study Tau protein that include basic and advanced methods and protocols from in vitro assays to in vivo models that address the molecular and functional aspects of tau physiopathology and many of its related technical issues. The chapters in this book are organized into five parts: Part One describes conformational and functional studies of native tau protein using wet and non-wet lab protocols. Part Two looks at in vitro methods to monitor or control the formation of Tau oligomers and fibrils, and the fibrillization process. Part Three provides protocols for the characterization and in vitro introduction of post-translational modifications in Tau protein for further functional studies. Part Four describes analytical tools for the detection of Tau proteins under various forms, factors associated with Tau pathology, and MAPT gene studies. Finally, Part Five explores cellular and in vivo models for the investigations of Tau physiopathology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Tau Proteins: Methods and Protocols, Second Edition is a valuable tool for any researcher interested in learning more about this important and developing field related to Tau protein as a relevant and attractive target for neurodegeneration therapies. 628 pp. Englisch.

Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume contains cutting-edge molecular biology methods on neuroprotective mechanisms and specific preclinical models of the CNS injury, iseases and planning translation. Chapters guide readers throughneuropathology, neuroprotection,Alzheimer's disease, amyotrophic lateral sclerosis, ALS, Huntington's disease , multiple sclerosis, Parkinson's disease, spinal cord injury, traumatic brain injury, and ischemic brain injury.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Neuroprotection: Methods and Protocolsaims to ensure successful results in the further study of this vital field. 664 pp. Englisch.

Buch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This updated edition explores essential techniques and newly emerged applications used in the expanding field of peptidomics. The opening chapters describe sample preparation and basic techniques. Subsequent chapters delve into quantitative peptidomics, bioinformatics, deep learning approaches, non-human model organisms, and peptide toxins. The last section includes multiple chapters on applications of peptidomics for human clinical specimens. Written for the highly successful Methods in Molecular Biology series, chapters include overviews and introductions to their respective topics, lists of the necessary materials, reagents, and instrumentation, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Peptidomics: Methods and Strategies, Second Edition serves as an ideal guide for state-of-the-art approaches in this vital field. 520 pp. Englisch.