New York: Humana Press, 2019. Very Good condition but for notes on the front endpapers, and notes and a small soiled spot on the next two pages. All other pages are clean and unmarked with NO underlining. NO highlighting. NO margin notes. With many illustrations, some in color. Molecular diagrams. Tables. Bibliographical references. Index. Volume # 1961 in the publisher's Methods in Molecular Biology series. For no apparent reason, someone has drawn a harmless circle on the front cover (2 inches in diameter). From the rear cover: "This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field.". First Edition. Hardcover. Very Good condition/No dust jacket, as issued. Illus. by NOT a library discard. xi, 362pp. Great Packaging, Fast Shipping.

Bestandsnummer des Verk�ufers 027944

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Bibliografische Details

Titel

CRISPR Gene Editing: Methods and Protocols

Verlag

Humana Press, New York

Erscheinungsjahr

2019

Sprache

Englisch

ISBN-10

1493991698

ISBN-13

9781493991693

Einband

Hardcover

Illustrator

NOT a library discard

Auflage

First Edition.

Zustand

Very Good condition

Zustand des Schutzumschlags

No dust jacket, as issued

Verk�uferkataloge

Science / Technology / Computers

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Inhaltsangabe

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field.

Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.

Von der hinteren Coverseite

Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.

��ber diesen Titel� kann sich auf eine andere Ausgabe dieses Titels beziehen.

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