Titel: Guide to Techniques in Mouse Development, Volume 225: Guide to Techniques in Mouse Development (Methods in Enzymology)
Verlag: Academic Press, NY
Erscheinungsjahr: 1993
Sprache: Englisch
ISBN-10: 0127364501
ISBN-13: 9780127364506
Einband: Spiral Bound
Illustrator: B/W Photos; Diagrams, charts, Etc
Auflage: 1st
Zustand: Very Good

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This volume provides, for the first time, an extensive compilation of those state-of-the-art methods that have revolutionized the use of mice as an experimental system. This volume is a unique source for detailed descriptions to be used in research by both experienced and novice researchers - Germ cells and embryos; Fertilization; Gene expression: messenger RNA, reporter genes, proteins, methylation; Gene identification; Nuclear transplantation; Transgenic animals: pronuclear injection, embryonic stem cells, and gene targeting; Lineage analysis.

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Guide to Techniques in Mouse Development, a volume designed to meet the needs of students, experienced researchers, and newcomers to the field, presents for the first time in a single source up-to-date technology used in research carried out with mice.
Key Features of the Guide
* Comprehensive molecular biological methods in mouse development compiled specifically for this volume
* Articles written by some of the most experienced researchers in the field of mouse development
Among the topics covered
* Gametes and embryos
* Transgenics
* Gene targeting
* Embryonic stem (ES) cells
* Microinjection
* Nuclear transplantation
* Reporter genes
* Gene cloning
* YAC libraries
* In situ hybridization

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