Craniofacial Development: Methods and Protocols: 2403 (Methods in Molecular Biology) - Hardcover

 
9781071618462: Craniofacial Development: Methods and Protocols: 2403 (Methods in Molecular Biology)

Inhaltsangabe

This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (c. elegans, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and in vivo cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using in-situ hybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR/Cas9-mediated deletion. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. 

Authoritative and cutting-edge, Craniofacial Development: Methods and Protocols aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques. 


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Über die Autorin bzw. den Autor

Charlotte y Peter Fiell son dos autoridades en historia, teoría y crítica del diseño y han escrito más de sesenta libros sobre la materia, muchos de los cuales se han convertido en éxitos de ventas. También han impartido conferencias y cursos como profesores invitados, han comisariado exposiciones y asesorado a fabricantes, museos, salas de subastas y grandes coleccionistas privados de todo el mundo. Los Fiell han escrito numerosos libros para TASCHEN, entre los que se incluyen 1000 Chairs, Diseño del siglo XX, El diseño industrial de la A a la Z, Scandinavian Design y Diseño del siglo XXI.

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This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (<i>c. elegans</i>, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and&nbsp;<i>in vivo</i>&nbsp;cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using&nbsp;<i>in-situ&nbsp;</i>hybridisation and&nbsp;single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as&nbsp;CRISPR/Cas9-mediated deletion.&nbsp;Written in the format of the highly successful&nbsp;<i>Methods in Molecular Biology&nbsp;</i>series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.&nbsp;<div><br>Authoritative and cutting-edge,&nbsp;<i>Craniofacial Development: Methods and Protocols</i>&nbsp;aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques.&nbsp;<p></p><div><br></div></div>

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9781071618493: Craniofacial Development: Methods and Protocols: 2403 (Methods in Molecular Biology)

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ISBN 10:  1071618490 ISBN 13:  9781071618493
Softcover