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Microinjection (Methods and Tools in Biosciences and Medicine) - Hardcover

 
9783764359737: Microinjection (Methods and Tools in Biosciences and Medicine)
Hardcover
ISBN 10: 3764359730 ISBN 13: 9783764359737
Verlag: Birkhäuser, 1999

Inhaltsangabe

Microinjection is an essential technique with a wide range of applications. Therefore, the book addresses a large audience. It features a shared European / American editorship and describes methodology and applications of microinjection, a powerful technique that is an indispensible approach today for a wide range of questions in cell and molecular biology. The book gives information on the technique's principles, the required equipment, preparation of receiving cells and injected material, analysis of the results, and then provides detailed protocols for a wide range of important applications in different cellular systems. Because of its outstanding relevance in cell biology a large part of the book is dedicated to the Xenopus laevis oocyte system.

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Reseña del editor

Microinjection is an essential technique with a wide range of applications. Therefore, the book addresses a large audience. It features a shared European / American editorship and describes methodology and applications of microinjection, a powerful technique that is an indispensible approach today for a wide range of questions in cell and molecular biology. The book gives information on the technique's principles, the required equipment, preparation of receiving cells and injected material, analysis of the results, and then provides detailed protocols for a wide range of important applications in different cellular systems. Because of its outstanding relevance in cell biology a large part of the book is dedicated to the Xenopus laevis oocyte system.

Reseña del editor

The technique of needle microinjection has become a prominent experimental approach in biological research. Cellular organelles, DNA and RNA, enzymes, structural proteins, metabolites, ions and antibodies are just some of the molecular and cellular elements that have been transposed from test tubes into living cells by needle injection. This technique is broadly used as a valuable tool for the study of many different cell responses in a variety of systems. This text presents information on the technique of needle microinjection's principles, the required equipment, preparation of receiving cells and injected material and the analysis of the results. It provides detailed protocols for a wide range of important applications and different cellular systems from the fields of cell cycle regulation, signal transduction, study of transcriptional regulations, cytoskeletal functions, secretion and intracellular transport.

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Verlag
Birkhäuser
Erscheinungsdatum
1999
Sprache
Englisch
ISBN 10 
3764359730
ISBN 13 
9783764359737
Einband
Tapa dura
Anzahl der Seiten
252
Herausgeber
Lacal Juan Carlos, Perona Rosario, Feramisco James
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