Microinjection (Methods and Tools in Biosciences and Medicine) - Hardcover

Hardcover

ISBN 10: 3764359730 ISBN 13: 9783764359737

Verlag: Birkhäuser, 1999

Inhaltsangabe

1. Needle Microinjection: A Brief History.- 2. Microinjection of Macromolecules into Mammalian Cells in Culture.- 3. The Use of Plasmid Microinjection to Study Specific Cell Cycle Phase Transitions.- 4. Combining Microinjection with Microanalytical Methods to Investigate Regulation of Metabolic Pathways.- 5. Import of Stably Folded Proteins into Peroxisomes.- 6. Study of the Function of Microtubule Proteins.- 7. Cytoskeleton Regulation by Rho Proteins.- 8. Cytoskeletal Regulation by Rho-p21 Associated Kinases: Analysis of ROK and PAK Function by Plasmid Microinjection.- 9. Use of Microinjection to Study Apoptosis and its Prevention.- 10. Role of the InsP3 Receptor in Intracellular Ca2+ Release and Ca2+ Entry.- 11. Microinjection of Xenopus laevis Oocytes: A Model System.- 12. Xenopus laevis Oocytes as a Model for Studying the Activation of Intracellular Kinases.- 13. Signal Transduction in Xenopus laevis Oocytes by Ras Oncoproteins and Lipid Metabolites.- 14. The Multiple Roles of Mos During Meiosis.- 15. Use of the Xenopus Oocyte System to Study RNA Transport.- 16. Functional Expression of G Protein-Coupled Receptors in Xenopus laevis Oocytes.- 17. Expression of Glucose Transporters in Xenopus laevis Oocytes: Applications for the Study of Membrane Transporter Function and Regulation.- 18. DNA Injection into Xenopus laevis Embryos as a Tool to Study Spatial Gene Activity.- 19. Transformation of Nematodes by Microinjection.- Guide to Solutions.- Guide to Protocols.- Troubleshooting Guide.

Über diesen Titel

Reseña del editor

Microinjection is an essential technique with a wide range of applications. Therefore, the book addresses a large audience. It features a shared European / American editorship and describes methodology and applications of microinjection, a powerful technique that is an indispensible approach today for a wide range of questions in cell and molecular biology. The book gives information on the technique's principles, the required equipment, preparation of receiving cells and injected material, analysis of the results, and then provides detailed protocols for a wide range of important applications in different cellular systems. Because of its outstanding relevance in cell biology a large part of the book is dedicated to the Xenopus laevis oocyte system.

Reseña del editor

The technique of needle microinjection has become a prominent experimental approach in biological research. Cellular organelles, DNA and RNA, enzymes, structural proteins, metabolites, ions and antibodies are just some of the molecular and cellular elements that have been transposed from test tubes into living cells by needle injection. This technique is broadly used as a valuable tool for the study of many different cell responses in a variety of systems. This text presents information on the technique of needle microinjection's principles, the required equipment, preparation of receiving cells and injected material and the analysis of the results. It provides detailed protocols for a wide range of important applications and different cellular systems from the fields of cell cycle regulation, signal transduction, study of transcriptional regulations, cytoskeletal functions, secretion and intracellular transport.

Details zur Ausgabe

• Verlag: Birkhäuser
• Erscheinungsdatum: 1999
• Sprache: Englisch
• ISBN 10: 3764359730
• ISBN 13: 9783764359737
• Einband: Tapa dura
• Anzahl der Seiten: 252
• Herausgeber: Feramisco James, Lacal Juan Carlos, Perona Rosario
• Kontakt zum Hersteller: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3,69115 Heidelberg, Germany; Heidelberg; 69115; Deutschland