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Hardcover. Zustand: gut. 2010 | 3rd ed. 2011. Fully revised and updated, Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. The text presents cutting-edge topics, such as polychromatic, quantitative, and high throughput flow cytometry, and more. With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users. Content: 1. Flow Cytometry: An Introduction Alice L. Givan 2. Breaking the Dimensionality Barrier C. Bruce Bagwell 3. Quantitative Fluorescence Measurements with Multicolor Flow Cytometry Lili Wang, Adolfas K. Gaigalas, and Ming Yan 4. Quantum Dots for Quantitative Flow Cytometry Yang Wu, Larry A. Sklar, and Tione Buranda 5. Bead-Based Multiplexed Analysis of Analytes by Flow Cytometry Henri C. van der Heyde and Irene Gramaglia 6. Flow-Based Combinatorial Antibody Profiling: An Integrated Approach to Cell Characterization Shane Bruckner, Ling Wang, Ruiling Yuan, Perry Haaland, and Amitabh Gaur 7. Tracking Immune Cell Proliferation and Cytotoxic Potential Using Flow Cytometry Joseph D. Tario, Jr., Katharine A. Muirhead, Dalin Pan, Mark E. Munson, and Paul K. Wallace 8. Multiparameter Intracellular Cytokine Staining Patricia Lovelace and Holden T. Maecker 9. Phospho Flow Cytometry Methods for Analysis of Kinase Signaling in Cell Lines and Primary Human Blood Samples Peter O. Krutzik, Angelica Trejo, Kenneth R. Schulz, and Garry P. Nolan 10. Multiparametric Analysis of Apoptosis by Flow Cytometry William G. Telford, Akira Komoriya, Beverly Z. Packard, and C. Bruce Bagwell 11. Multiparameter Cell Cycle Analysis James W. Jacobberger, R. Michael Sramkoski, and Tammy Stefan 12. Rare Event Detection and Analysis in Flow Cytometry: Bone Marrow Mesenchymal Stem Cells, Breast Cancer Stem/Progenitor Cells in Malignant Effusions, and Pericytes in Disaggregated Adipose Tissue Ludovic Zimmerlin, Vera S. Donnenberg, and Albert D. Donnenberg 13. Flow Cytometric-Based Identification of Immature Myeloerythroid Development Cornelis J.H. Pronk and David Bryder 14. Flow Cytometry Immunophenotyping of Hematolymphoid Neoplasia Katherine R. Calvo, Catharine S. McCoy, and Maryalice Stetler-Stevenson 15. Flow Cytometry Assays in Primary Immunodeficiency Diseases Maurice R.G. O'Gorman, Joshua Zollett, and Nicolas Bensen 16. Flow Cytometric Analysis of Microparticles Henri C. van der Heyde, Irene Gramaglia, Valery Combes, Thaddeus C. George, and Georges E. Grau 17. Noncytotoxic DsRed Derivatives for Whole-Cell Labeling Rita L. Strack, Robert J. Keenan, and Benjamin S. Glick 18. Flow Cytometric FRET Analysis of Protein Interaction Gyoergy Vereb, Peter Nagy, and Janos Szoellosi 19. Fluorescent Protein-Assisted Purification for Gene Expression Profiling M. Raza Zaidi, Chi-Ping Day, and Glenn Merlino 20. Multiparametric Analysis, Sorting, and Transcriptional Profiling of Plant Protoplasts and Nuclei According to Cell Type David W. Galbraith, Jaroslav Janda, and Georgina M. Lambert 21. Lentiviral Fluorescent Protein Expression Vectors for Biotinylation Proteomics Irene Riz, Teresa S. Hawley, and Robert G. Hawley 22. Standard Practice for Cell Sorting in a BSL-3 Facility Stephen P. Perfetto, David R. Ambrozak, Richard Nguyen, Mario Roederer, Richard A. Koup, and Kevin L. Holmes 23. The Cytometric Future: It Ain't Necessarily Flow! Howard M. Shapiro Reihe/Serie Methods in Molecular Biology ; 699 Zusatzinfo 26 Tables, black and white; XII, 486 p. Verlagsort Totowa, NJ Sprache englisch Maße 178 x 254 mm Studium 1. Studienabschnitt Vorklinik Histologie Embryologie Studium 2. Studienabschnitt Klinik Anamnese Körperliche Untersuchung Naturwissenschaften Biologie Genetik Molekularbiologie Technik Umwelttechnik Biotechnologie ISBN-10 1-61737-949-2 1617379492 ISBN-13 978-1-61737-949-9 9781617379499 Flow Cytometry Protocols Methods in Molecular Biology Band 699 [Hardcover] Hawley, Teresa S. Hawley, Robert In englischer Sprache. 485 pages. 178 x 254 mm.…

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Zustand: New. During the past three decades, the sugar moiety of complex carbohydrates has been found to be involved in important interactions of immunological specificity of antigens and to participate in a variety of cellular functions. The long polysaccharide side cha.

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Zustand: Gut. Zustand: Gut | Seiten: 868 | Sprache: Englisch | Produktart: Bücher | During the past three decades, the sugar moiety of complex carbohydrates has been found to be involved in important interactions of immunological specificity of antigens and to participate in a variety of cellular functions. The long polysaccharide side chains of the lipopolysaccharides on the outer membrane of Gram negative organisms provide surface antigens for differential serodiagnosis. Bacterial surface lectins are important in mediating the attachment of bacteria to host cells in the of infectious diseases. The carbohydrate pathogenesis moieties of cell surface glycoconjugates (glycoproteins and glycolipids) of mammals are the sites for intercellular recognition and for the regulatory molecular interactions such as interaction of complex carbohydrate with hormones or hepatic lectins. The carbohydrate side chains of many complex carbohydrates play essential roles as antigenic determinants b of human blood group ABH, Lea, Le , I, and i activities, as the Forssman specific determinant, and as tumor associated antigenic determinants. Prompted by these and other advances in the field, a Symposium on Molecular Immunology of Complex Carbohydrates was organized as a satellite meeting of the 8th International Glycoconjugate Conference held on September 8- 13, 1985, in Houston, Texas, U. S . A. Many eminent scientists contributed their knowledge at this meeting. The lecture and poster materials of the symposium are contained in this proceeding book, which is divided into four Sections and one Appendix. Section I is entitled Antibody Specificity, Epitope, and Lectinology. Dr. Elvin A.…

Taschenbuch. Zustand: Neu. Neuware - During the past three decades, the sugar moiety of complex carbohydrates has been found to be involved in important interactions of immunological specificity of antigens and to participate in a variety of cellular functions. The long polysaccharide side chains of the lipopolysaccharides on the outer membrane of Gram negative organisms provide surface antigens for differential serodiagnosis. Bacterial surface lectins are important in mediating the attachment of bacteria to host cells in the of infectious diseases. The carbohydrate pathogenesis moieties of cell surface glycoconjugates (glycoproteins and glycolipids) of mammals are the sites for intercellular recognition and for the regulatory molecular interactions such as interaction of complex carbohydrate with hormones or hepatic lectins. The carbohydrate side chains of many complex carbohydrates play essential roles as antigenic determinants b of human blood group ABH, Lea, Le , I, and i activities, as the Forssman specific determinant, and as tumor associated antigenic determinants. Prompted by these and other advances in the field, a Symposium on Molecular Immunology of Complex Carbohydrates was organized as a satellite meeting of the 8th International Glycoconjugate Conference held on September 8- 13, 1985, in Houston, Texas, U. S . A. Many eminent scientists contributed their knowledge at this meeting. The lecture and poster materials of the symposium are contained in this proceeding book, which is divided into four Sections and one Appendix. Section I is entitled Antibody Specificity, Epitope, and Lectinology. Dr. Elvin A.…

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Taschenbuch. Zustand: Neu. The Molecular Immunology of Complex Carbohydrates -2 | Albert M. Wu | Taschenbuch | xxiv | Englisch | 2013 | Springer | EAN 9781461354697 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu. …

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Buch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - SECTION I Carbohydrate-Protein Interactions: Plant and Animal Lectins.- I-1 The Structural Basis for Carbohydrate Recognition by Lectins.- I-2 Lectin Cross-linking Interactions with Multivalent Carbohydrates.- I-3 Classification of Plant Lectins in Families of Structurally and Evolutionary Related Proteins.- I-4 Expression of Binding Properties of Gal/Ga1NAc Reactive Lectins by Mammalian Glycotopes.- I-5 'Glyco-Epitope' Assignments for the Selectins: Advances Enabled by the Neoglycolipid Technology in Conjunction with Synthetic Carbohydrate Chemistry.- I-6 Animal Lectins: From Initial Description to Elaborated Structural and Functional Classification.- I-7 Galectins and Other Endogenous Carbohydrate-Binding Proteins of Animal Bladder.- I-8 Binding Properties and Applications of Aplysia Gonad Lectin.- I-9 Lectin and Anti-carbohydrate Antibody Assays Using Chemically Modified Ligands.- SECTION II Structure and Antigenicity of Glycoconjugates.- II-1 Intramolecular Carbohydrate-Protein Interaction.- II-2 Red Blood Cell Antigens Responsible for Inherited Types of Polyagglutination.- II-3 Antigenic Properties of Human Glycophorins-An Update.- II-4 KDN-Containing Glycoprotein from Loach Skin Mucus.- II-5 Glycoconjugates from Parasitic Helminths: Structure Diversity and Immunobiological Implications.- II-6 Glycosyl Phosphatidylinositol-Linked Glycoconjugates: Structure, Biosynthesis, and Function.- II-7 Enzymatic and Chemical Approaches for the Synthesis of Sialyl Glycoconjugates.- SECTION III Glycotopes Expression, Metabolism, and Functions.- III-1 The Genetic Regulation of Fucosylated and Sialylated Antigens on Developing Myeloid Cells.- III-2 Transcriptional Regulation of Expression of Carbohydrate Ligands for Cell Adhesion Molecules in the Selectin Family.- III-3Biosynthesis and Function of 1,6 Branched Mucin-Type Glycans.- III-4 Differentiation-Associated Expression of ß-N-acetylgalactosaminylated N-linked Oligosaccharides in Mammary Epithelial Cells.- III-5 Metabolism and Role of O-Acetylated Sialic Acids.- III-6 Contradictory Functions of the Sulfatide in the Blood Coagulation System.- III-7 Interaction of GM2 Activator Protein with Glycosphingolipids.- SECTION IV Complex Carbohydrates in Development and Treatment of Cancer.- IV-1 Tumor-Associated Carbohydrate Antigens Defining Tumor Malignancy: Basis for Development of Anti-cancer Vaccines.- IV-2 Carbohydrate-Mediated Adhesion of Human Colon Carcinoma Cells to Human Liver Sections.- IV-3 O-Linked N-Acetylglucosamine and Cancer: Messages from the Glycosylation of c-Myc.- IV-4 Analysis of a Murine Anti-Ganglioside GD2 Monoclonal Antibody Expressing Both IgG2a and IgG3 Isotypes: Monoclonality, Apoptosis Triggering, and Activation of Cellular Cytotoxicity on Human Melanoma Cells.- SECTION V Complex Carbohydrates in Microbial and Viral Infections and Vaccine Design.- V-1 Pathogen-Host Protein-Carbohydrate Interactions as the Basis of Important Infections.- V-2 Host Mediated Variation and Receptor Binding Specificity of Influenza Viruses.- V-3 Bacterial Polysaccharides as Vaccines - Immunity and Chemical Characterization.- V-4 The Conformational Epitope of Type III Group B Streptococcus Capsular Polysaccharide.- V-5 T-independent IgA Responses to Microbial Polysaccharides.- V-6 Protective Immunity and Gene Expression Related to Pneumococcal Glycoconjugates.- V-7 Structure and Functional Genomics of Lipopolysaccharide Expression in Haemophilus influenzae.- V-8 Molecular Mimicry of Host Structures by Lipooligosaccharides of Neisseria meningitidis: Characterization ofSialylated and Nonsialylated Lacto-N-Neotetraose (Gal 1-4G1cNAc 1-3Gal 1-4Glc) Structures in Lipooligosaccharides using Monoclonal Antibodies and Specific Lectins.- V-9 NMR and Molecular Modeling of Complex Carbohydrates and Carbohydrate-Protein Interactions: Applications to Anti-bacteria Vaccines.- Append.- A-I A Guide to the Carbohydrate Specificities of Applied Lectin.…

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Buch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - This book covers the expanding landscape of glycoscience over the past decade from the earlier books titled The Molecular Immunology of Complex Carbohydrates 1, 2, and 3 . It aims to provide systematic and comprehensive reviews of the glycoscience field. In addition to the diverse roles of glycans in the immune system, this volume broadens its scope to encompass (i) human glycoscience across multiple systems of the human body most notably the nervous system, influencing both physiological processes like neuronal axon generation and pathological conditions like Alzheimer's disease, (ii) microbial glycoscience, and (iii) environmental glycoscience.Moreover, significant attention is given to the current and emerging (iv) applications of glycoscience in biotechnology and chemical technology. Comprised of 36 chapters and authored by 89 internationally recognized experts in addition to one author, who worked on the proofs of his late mother, this book provides a wide-ranging and in-depth exploration of glycan biosynthesis and structure, extending to their functional significance in various human biological systems and microbial physiology. It offers detailed insights into complex biological interactions not only cell cell recognition and communication, but also the dynamic relationships between host and microbe, human and food or pollen, and even cross-reactivities between foods and pollens. This book highlights modern biotechnology and chemical innovation in glycan-based and glycan-binding protein (lectin)-based syntheses and applications of glycans and lectins in detection, diagnosis, prevention, and therapy of physiological and pathogenic diseases. Cutting-edge examples of glycan- and lectin-based strategies in vaccine development and biomedical research are also thoroughly presented. Enriched with numerous explanatory illustrations and extensively referenced with foundational and recent literatures, this work constitutes a valuable resource for students, early-career scientists, researchers, clinicians, and specialists engaged in the life sciences and medicine.…

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