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Taschenbuch. Zustand: Neu. Characterization Of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase | Human HMG-CoA Reductase Protein Expression | Khush Bakhat Samreen (u. a.) | Taschenbuch | 104 S. | Englisch | 2014 | LAP LAMBERT Academic Publishing | EAN 9783846554173 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu.
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Taschenbuch. Zustand: Neu. Charakterystyka reduktazy 3-hydroksy-3-metyloglutarylo-koenzymu A | Ekspresja ludzkiego bia¿ka reduktazy HMG-CoA | Khush Bakhat Samreen (u. a.) | Taschenbuch | Polnisch | 2026 | Wydawnictwo Nasza Wiedza | EAN 9786209982507 | Verantwortliche Person für die EU: SIA OmniScriptum Publishing, Brivibas Gatve 197, 1039 RIGA, LETTLAND, customerservice[at]vdm-vsg[dot]de | Anbieter: preigu.
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Taschenbuch. Zustand: Neu. Caracterização da 3-hidroxi-3-metilglutaril-coenzima A redutase | Expressão da proteína HMG-CoA redutase humana | Khush Bakhat Samreen (u. a.) | Taschenbuch | Portugiesisch | 2026 | Edições Nosso Conhecimento | EAN 9786209985065 | Verantwortliche Person für die EU: SIA OmniScriptum Publishing, Brivibas Gatve 197, 1039 RIGA, LETTLAND, customerservice[at]vdm-vsg[dot]de | Anbieter: preigu.
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Taschenbuch. Zustand: Neu. Charakterisierung der 3-Hydroxy-3-methylglutaryl-Coenzym-A-Reduktase | Expression des menschlichen HMG-CoA-Reduktase-Proteins | Khush Bakhat Samreen (u. a.) | Taschenbuch | 84 S. | Deutsch | 2026 | Verlag Unser Wissen | EAN 9786209972263 | Verantwortliche Person für die EU: SIA OmniScriptum Publishing, Brivibas Gatve 197, 1039 RIGA, LETTLAND, customerservice[at]vdm-vsg[dot]de | Anbieter: preigu.
Sprache: Englisch
Verlag: LAP LAMBERT Academic Publishing Feb 2014, 2014
ISBN 10: 3846554170 ISBN 13: 9783846554173
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -HMG-CoA reductase catalyzes the conversion of HMG-CoA to mevalonate, which is known as the rate-limiting step in the biosynthesis of cholesterol and other intermediates of mevalonate pathway.The purified plasmid (pGEXtev/hmgr) containing gene of human HMG-CoA reductase was confirmed by restriction analysis and sequencing. After verification, it was transformed into competent cells of Escherchia coli (BL21 and DH5 ) for which DH5 produced most promising results. Protein expression was induced with IPTG and further optimized through varying the concentration of IPTG, betain, sorbitol and addition of various buffers. The influence of various temperatures on protein expression was also investigated. The gene of interest (hmgr) was sub cloned into pET 22b. Sub cloned plasmid pET22b/hmgr was induced in E. coli BL21and its expression was optimized by employing different IPTG concentrations and taking samples at different ODs (A600). The plasmid pGEXtev/hmgr in DH5 expresses a protein of 54 kDa along with GST fusion tag. The protein consists of 283 amino acids and had a molecular weight of 31.1 kDa and theoretical isoelectric point is 7.68. 104 pp. Englisch.
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In den WarenkorbZustand: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Manzoor FarkhandaDr. Farkhanda Manzoor has done MSc. (Zoology), PhD and Post doctorate (Entomology) from Ohio State University, US. She has 45 publications in her record. Ms. Khush Bakhat Samreen has done BS (Zoology) from Lahore Col.
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In den WarenkorbZustand: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Samreen Khush BakhatKhush Bakhat Samreen had done her M-phill in Biotechnology from Lahore College for Women Univrsity, Lahore, Pakistan. She has publications in the form of book and paper. Her specific area of research was Molecular.
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Verlag: VDM Verlag Dr. Mueller Aktiengesellschaft & Co. KG, 2014
ISBN 10: 3846554170 ISBN 13: 9783846554173
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Verlag: LAP LAMBERT Academic Publishing Feb 2014, 2014
ISBN 10: 3846554170 ISBN 13: 9783846554173
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Taschenbuch. Zustand: Neu. This item is printed on demand - Print on Demand Titel. Neuware -HMG-CoA reductase catalyzes the conversion of HMG-CoA to mevalonate, which is known as the rate-limiting step in the biosynthesis of cholesterol and other intermediates of mevalonate pathway.The purified plasmid (pGEXtev/hmgr) containing gene of human HMG-CoA reductase was confirmed by restriction analysis and sequencing. After verification, it was transformed into competent cells of Escherchia coli (BL21 and DH5¿) for which DH5¿ produced most promising results. Protein expression was induced with IPTG and further optimized through varying the concentration of IPTG, betain, sorbitol and addition of various buffers. The influence of various temperatures on protein expression was also investigated. The gene of interest (hmgr) was sub cloned into pET 22b. Sub cloned plasmid pET22b/hmgr was induced in E. coli BL21and its expression was optimized by employing different IPTG concentrations and taking samples at different ODs (A600). The plasmid pGEXtev/hmgr in DH5¿ expresses a protein of 54 kDa along with GST fusion tag. The protein consists of 283 amino acids and had a molecular weight of 31.1 kDa and theoretical isoelectric point is 7.68.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 104 pp. Englisch.