Cytochrome p450 protocols (69 Ergebnisse)

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softcover. Zustand: good. no jacket. 0. Humana Press, 2021. Oversize paperback. 379pp. Some creasing to spine & covers. Clean pages. Good+.

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Taschenbuch. Zustand: Neu. Cytochrome P450 Protocols | Ian R. Phillips (u. a.) | Taschenbuch | xii | Englisch | 2016 | Humana | EAN 9781493962693 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu. …

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Taschenbuch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - This collection explores detailed experimental protocols necessary for setting up a variety of in vitro cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline. Major factors affecting drug metabolism include CYP expression levels, kinetic parameters for individual CYP enzymes, CYP inhibition and induction, time-dependent inhibition (TDI), CYP stability, non-CYP stability, UDP-glucuronosyltransferases (UGT) stability, excretion mechanisms, and drug-drug interactions (DDI), all addressed in this volume. Written for the Methods in Pharmacology and Toxicology series, chapters include helpful background information on the in vitro assay, a list of all the materials, reagents, and equipment necessary to carry out the assay, a step-by-step protocol, notes containing common and unexpected experimental problems in the assay, as well as references containing important supplementary reading.Authoritativeand practical, Cytochrome P450: In Vitro Methods and Protocols serves as a key guide for researchers in the area of discovery and development of new medicines.…

Taschenbuch. Zustand: Neu. Cytochrome P450 | In Vitro Methods and Protocols | Zhengyin Yan (u. a.) | Taschenbuch | Methods in Pharmacology and Toxicology | xi | Englisch | 2022 | Humana | EAN 9781071615447 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu.…

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Zustand: New. Editor(s): Phillips, Ian R. Series: Methods in Molecular Biology. Num Pages: 364 pages, 47 black & white illustrations, biography. BIC Classification: PSB. Category: (P) Professional & Vocational. Dimension: 227 x 152 x 25. Weight in Grams: 636. . 2010. 2nd ed. 2006. Paperback. . . . .

Zustand: New. This book provides a wide range of techniques accessible to researchers in biochemistry, molecular biology, pharmacology, toxicology, environmental biology and genetics. It features step-by-step, readily reproducible protocols and notes on troubleshooting. Editor(s): Phillips, Ian R.; Shephard, Elizabeth A.; Montellano, Paul R.Ortiz de. Series: Methods in Molecular Biology. Num Pages: 319 pages, 19 black & white tables, biography. BIC Classification: PSBC. Category: (P) Professional & Vocational. Dimension: 261 x 182 x 23. Weight in Grams: 816. . 2013. 3rd ed. 2013. hardcover. . . . . …

Zustand: New. Presenting the techniques that use the methodologies for the investigation of P450s, this title includes protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases, expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Series: Methods in Molecular Biology. Num Pages: 364 pages, 47 black & white illustrations, biography. BIC Classification: MMGT; PSB; PSD. Category: (P) Professional & Vocational. Dimension: 240 x 162 x 28. Weight in Grams: 714. . 2005. 2nd ed. 2006. Hardback. . . . .…

Taschenbuch. Zustand: Neu. Cytochrome P450 Protocols | Ian R Phillips | Taschenbuch | xiv | Englisch | 2010 | Humana Press | EAN 9781617375729 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu. …

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Taschenbuch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source. For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Also described are techniques for the generation of mice with targeted gene disruptions. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source. Each chapter is written by researchers who have been involved in the development and application of the particular technique to P450s or FMOs. The protocols are presented in a step-by-step manner, with extensive cross-references to notes that highlight critical steps, potential problems, and alternative methods so that the researcher can understand the basis of the method and perform it successfully. Cutting-edge and highly practical, Cytochrome P450, Second Edition provides both novices and experienced researchers across many fields all the tools needed today to elucidate the crucial biological role played by cytochrome P450 in the metabolism of therapeutic drugs, chemical carcinogens, and environmental pollutants.…